TY - JOUR
T1 - Application of CRISPR/Cas9 to the study of brain development and neuropsychiatric disease
AU - Powell, S. K.
AU - Gregory, J.
AU - Akbarian, S.
AU - Brennand, K. J.
N1 - Funding Information:
Kristen Brennand is a New York Stem Cell Foundation - Robertson Investigator. This work was partially supported by National Institutes of Health (NIH) grants R01 MH101454 (KJB) and R01 MH106056 (SA) and P50 MH096890 (SA), as well as the New York Stem Cell Foundation and the Brain & Behavior Research Foundation.
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/7
Y1 - 2017/7
N2 - CRISPR/Cas9 technology has transformed our ability to manipulate the genome and epigenome, from efficient genomic editing to targeted localization of effectors to specific loci. Through the manipulation of DNA- and histone-modifying enzyme activities, activation or repression of gene expression, and targeting of transcriptional regulators, the role of gene-regulatory and epigenetic pathways in basic biology and disease processes can be directly queried. Here, we discuss emerging CRISPR-based methodologies, with specific consideration of neurobiological applications of human induced pluripotent stem cell (hiPSC)-based models.
AB - CRISPR/Cas9 technology has transformed our ability to manipulate the genome and epigenome, from efficient genomic editing to targeted localization of effectors to specific loci. Through the manipulation of DNA- and histone-modifying enzyme activities, activation or repression of gene expression, and targeting of transcriptional regulators, the role of gene-regulatory and epigenetic pathways in basic biology and disease processes can be directly queried. Here, we discuss emerging CRISPR-based methodologies, with specific consideration of neurobiological applications of human induced pluripotent stem cell (hiPSC)-based models.
UR - http://www.scopus.com/inward/record.url?scp=85019739751&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2017.05.007
DO - 10.1016/j.mcn.2017.05.007
M3 - Review article
C2 - 28549865
AN - SCOPUS:85019739751
SN - 1044-7431
VL - 82
SP - 157
EP - 166
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
ER -