TY - JOUR
T1 - Appearance of Epstein-Barr virus-associated antigens in infected Raji cells
AU - Gergely, Lajos
AU - Klein, George
AU - Ernberg, Ingemar
N1 - Funding Information:
This work was supported by grants from the Swedish Cancer Societ.y, NH1 Contract No. 69-9005 wit.hin the Specinl Virus-Cancer Program of the National Cancer Institute, NIII, PIIS, Lotten Bohrnan’s Fund, Magnus Bergvall’s Foundation and the Jnne Coffin Childs Memorial Fund for Medical Research.
PY - 1971/7
Y1 - 1971/7
N2 - The appearance of EBV-associated early and membrane antigens (EA and MA) was studied in EBV-infected Raji cells. Newly synthetized MA appeared on approximately 50% of the cells 20-24 hours after infection. Around the same time intranuclear EA appeared in 2-5% of the cells, spreading later into the cytoplasm. The appearance of MA and EA was not inhibited by Ara C and iododeoxyuridine, but was completely prevented by puromycin. Viral capsid antigen (VCA) synthesis was not observed during an observation period of 4 days. Unless DNA synthesis was inhibited, the frequency of MA- and EA-positive cells decreased continuously after 1 or 2 days; the infectious cycle was thus abortive. Exposure of EBV to sera with high anti-MA titers neutralized the virus, as judged by its inability to induce EA. The virusneutralizing titers could be reduced by absorbing the sera with an EBV carrying cell line containing a high frequency of MA-positive cells, but not with a line with few MA-positives. The frequency of VCA + EA + cells was approximately equal in both lines. This supports the hypothesis that MA may represent viral envelope components inserted into the cell membrane, in analogy with the HSV system.
AB - The appearance of EBV-associated early and membrane antigens (EA and MA) was studied in EBV-infected Raji cells. Newly synthetized MA appeared on approximately 50% of the cells 20-24 hours after infection. Around the same time intranuclear EA appeared in 2-5% of the cells, spreading later into the cytoplasm. The appearance of MA and EA was not inhibited by Ara C and iododeoxyuridine, but was completely prevented by puromycin. Viral capsid antigen (VCA) synthesis was not observed during an observation period of 4 days. Unless DNA synthesis was inhibited, the frequency of MA- and EA-positive cells decreased continuously after 1 or 2 days; the infectious cycle was thus abortive. Exposure of EBV to sera with high anti-MA titers neutralized the virus, as judged by its inability to induce EA. The virusneutralizing titers could be reduced by absorbing the sera with an EBV carrying cell line containing a high frequency of MA-positive cells, but not with a line with few MA-positives. The frequency of VCA + EA + cells was approximately equal in both lines. This supports the hypothesis that MA may represent viral envelope components inserted into the cell membrane, in analogy with the HSV system.
UR - http://www.scopus.com/inward/record.url?scp=0015098429&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(71)90107-3
DO - 10.1016/0042-6822(71)90107-3
M3 - Article
C2 - 4329584
AN - SCOPUS:0015098429
SN - 0042-6822
VL - 45
SP - 10
EP - 21
JO - Virology
JF - Virology
IS - 1
ER -