Apical Na+ conductance in maturing rabbit principal cell

Lisa M. Satlin, Lawrence G. Palmer

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67 Scopus citations

Abstract

Net Na+ absorption in microperfused rabbit cortical collecting ducts (CCDs) is low during the 1st wk of postnatal life, increasing substantially thereafter [L. M. Satlin. Am. J. Physiol. 266 (Renal Fluid Electrolyte Physiol. 35): F57-F65, 1994). To establish whether the low rate of Na+ absorption observed immediately after birth is due to a low apical Na+ permeability of the neonatal principal cell, we used the patch-clamp technique in split-open CCDs isolated from maturing rabbits to estimate conductance, number (N), and open probability (P) of apical Na4 channels in principal cells. With LiCl in the pipette and a NaCl or potassium gluconate solution, warmed to 37°C, in the bath, inward currents with a conductance of ∼11 pS (n = 23) were observed in 17% of cell-attached patches at 1 wk, 41% of patches at 2 wk, and 437< of patches at 5 wk. The mean N per patch in the 1st wk (0.22 ±0.09; n = 36) was significantly less than that observed in the 2nd (1.38 ±0.39; n = 34) and 5th (1.24 ±0.37; n = 21) wk of life. P, studied at positive pipette voltages, was significantly lower in the 1st wk (0.085 ±0.035; n = 5) than in the 2nd wk (0.345 ±0.063; n = 9) and 5th wk (0.291 ±0.058; n = 4). To confirm that the 11-pS channel represented the amiloride-sensitive apical Na+ channel, cell-attached patches in CCDs isolated from 2-wk-old rabbits were studied with 0.5 μM amiloride added to the LiCl pipette solution. Amiloride led to >90% reductions in mean open and closed times of the 11-pS conductance, consistent with blockade of the channel. These data indicate that N and P0 of apical amiloride-sensitive Na channels in principal cells increase significantly after birth.

Original languageEnglish
Pages (from-to)F391-F397
JournalAmerican Journal of Physiology
Volume270
Issue number3 PART 2
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • Amiloride
  • Cortical collecting duct
  • Development
  • Maxi-potassium ion channel
  • Patch clamp

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