Antistaphylococcal nanocomposite films based on enzyme - Nanotube conjugates

Ravindra C. Pangule, Sarah J. Brooks, Cerasela Zoica Dinu, Shyam Sundhar Bale, Sharon L. Salmon, Guangyu Zhu, Dennis W. Metzger, Ravi S. Kane, Jonathan S. Dordick

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

Infection with antibiotic-resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) is one of the primary causes of hospitalizations and deaths. To address this issue, we have designed antimicrobial coatings incorporating carbon nanotube - enzyme conjugates that are highly effective against antibiotic-resistant pathogens. Specifically, we incorporated conjugates of carbon nanotubes with lysostaphin, a cell wall degrading enzyme, into films to impart bactericidal properties against Staphylococcus aureus and Staphylococcus epidermidis. We fabricated and characterized nanocomposites containing different conjugate formulations and enzyme loadings. These enzyme-based composites were highly efficient in killing MRSA (>99% within 2 h) without release of the enzyme into solution. Additionally, these films were reusable and stable under dry storage conditions for a month. Such enzyme-based film formulations may be used to prevent growth of pathogenic and antibiotic-resistant microorganisms on various common surfaces in hospital settings. Polymer and paint films containing such antimicrobial conjugates, in particular, could be advantageous to prevent risk of staphylococcal-specific infection and biofouling.

Original languageEnglish
Pages (from-to)3993-4000
Number of pages8
JournalACS Nano
Volume4
Issue number7
DOIs
StatePublished - 27 Jul 2010
Externally publishedYes

Keywords

  • antimicrobial film
  • carbon nanotube
  • lysostaphin
  • methicillin-resistant Staphylococcus aureus (MRSA)
  • nanocomposite

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