Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1

Vidadi Yusibov, Anna Modelska, Klaudia Steplewski, Michail Agadjanyan, David Weiner, D. Craig Hooper, Hilary Koprowski

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

The coat protein (CP) of alfalfa mosaic virus was used as a carrier molecule to express antigenic peptides from rabies virus and HIV. The antigens were separately cloned into the reading frame of alfalfa mosaic virus CP and placed under the control of the subgenomic promoter of tobacco mosaic virus CP in the 30BRz vector. The in vitro transcripts of recombinant virus with sequences encoding the antigenic peptides were synthesized from DNA constructs and used to inoculate tobacco plants. The plant-produced protein (virus particles) was purified and used for immunization of mice. Both antigens elicited specific virus-neutralizing antibodies in immunized mice.

Original languageEnglish
Pages (from-to)5784-5788
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number11
DOIs
StatePublished - 27 May 1997
Externally publishedYes

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