TY - JOUR
T1 - Antigenic variants of influenza viruses
T2 - Marked differences in the frequencies of variants selected with different monoclonal antibodies
AU - Lubeck, Michael D.
AU - Schulman, Jerome L.
AU - Palese, Peter
N1 - Funding Information:
ACKNOWLEDGMENTS This study was supportedb y Public Health Service Grants AI-11823 and 81-14053f rom the National Institute of Allergy and InfectiousD iseases,b y Grant PCM-76-11066fr om the National ScienceF oundation, and by Grant MV-23A from the American Cancer Society. M.D.L. received fellowship support from the National Institute of Allergy and Infectious Diseases (AI-05664-OlAl) and from the New York Lung Association. P.P. is the recipient of an I. T. Hirsch1 Career Scientist Award. The authors thank Marlene Lin for her excellentt echnicala ssistance.T he P3 X-63 Ag8 myelomac ell line was kindly provided by Dr. W. Gerhard of the Wistar Institute, Philadelphia.
PY - 1980/4/30
Y1 - 1980/4/30
N2 - Monoclonal antibodies against the hemagglutinin of two influenza A viruses and one influenza B virus were used to analyze the frequencies of antigenic variant subpopulations in cloned virus seeds. Antigenic variants selected with monoclonal antibodies specific for different antigenic determinants were obtained over a wide range of frequencies. With one monoclonal antibody directed to the hemagglutinin of A/PR/8/34 virus antigenic variants were isolated at a frequency of 10-4.4, whereas another monoclonal antibody, specific for the hemagglutinin of X-31 virus, completely neutralized all infectious virus in the X-31 virus seeds examined, indicating a frequency of antigenic variation, for one seed at least, of less than 10-8.1.
AB - Monoclonal antibodies against the hemagglutinin of two influenza A viruses and one influenza B virus were used to analyze the frequencies of antigenic variant subpopulations in cloned virus seeds. Antigenic variants selected with monoclonal antibodies specific for different antigenic determinants were obtained over a wide range of frequencies. With one monoclonal antibody directed to the hemagglutinin of A/PR/8/34 virus antigenic variants were isolated at a frequency of 10-4.4, whereas another monoclonal antibody, specific for the hemagglutinin of X-31 virus, completely neutralized all infectious virus in the X-31 virus seeds examined, indicating a frequency of antigenic variation, for one seed at least, of less than 10-8.1.
UR - http://www.scopus.com/inward/record.url?scp=0018865387&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(80)90114-2
DO - 10.1016/0042-6822(80)90114-2
M3 - Article
C2 - 6154380
AN - SCOPUS:0018865387
SN - 0042-6822
VL - 102
SP - 458
EP - 462
JO - Virology
JF - Virology
IS - 2
ER -