Antigenic determinants of the 70,000 molecular weight glycoprotein of woolly monkey type C RNA virus

S. Hino, J. R. Stephenson, S. A. Aaronson

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The 70,000 molecular weight glycoprotein (gp70) of a type C RNA virus originally isolated from a woolly monkey has been partially purified and immunologically characterized. Evidence that this viral protein is viral coded was derived from studies showing its antigenic properties to be unaltered by virus passage in cells of different species. A broadly reactive competition immunoassay was developed utilizing antiserum prepared against feline leukemia virus to precipitate 125I labeled woolly monkey virus gp70. Gibbon and woolly viruses, as well as feline and several mouse type C viruses, all reacted with equal efficiency in this assay. In contrast, an endogenous virus of the baboon failed to cross react, suggesting that viruses of this latter group are less immunologically related to the others. In a homologous competition immunoassay for the woolly viral glycoprotein, the woolly virus was readily distinguished from otherwise closely related viruses of gibbon apes. These findings demonstrate the pronounced type specific antigenic determinants possessed by this viral protein. The antigenic determinants of gp70 responsible for neutralization have also been investigated.

Original languageEnglish
Pages (from-to)922-927
Number of pages6
JournalJournal of Immunology
Issue number4
StatePublished - 1975
Externally publishedYes


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