Antigen stimulation induces HIV envelope gp120-specific CD4+ T cells to secrete CCR5 ligands and suppress HIV infection

Gurvinder Kaur, Michael Tuen, Diana Virland, Sandra Cohen, Narinder K. Mehra, Christian Münz, Sayed Abdelwahab, Alfredo Garzino-Demo, Catarina E. Hioe

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


CD4+ T cells are critical for effective immune responses against HIV, but they are also the main cell type targeted by the virus. To investigate the key factors that could protect these cells from infection, we evaluated the capacity of HIV gp120-specific human CD4+ T cells to produce chemokines that inhibit HIV and determined their contribution in suppressing infection in the cells. Antigen stimulation of the CD4+ T cells elicited production of high amounts of CCR5 chemokines MIP-1α (CCL3), MIP-1β (CCL4), and RANTES (CCL5). Production of these CCR5 ligands was more readily and reproducibly detected than that of IFN-γ or IL-2. Importantly, in association with secretion of the CCR5 ligands, antigen stimulation made these CD4+ T cells more resistant to CCR5-tropic HIV-1. Conversely, in the absence of antigen stimulation, the cells were readily infected by the virus, and after infection, their capacity to produce MIP-1β and IFN-γ rapidly declined. Thus, vaccines that trigger HIV-specific CD4+ T cells to elicit robust and rapid production of anti-viral chemokines would be advantageous. Such responses would protect virus-specific CD4+ T cells from HIV infection and preserve their critical functions in mounting and maintaining long-lasting immunity against the virus.

Original languageEnglish
Pages (from-to)214-225
Number of pages12
Issue number1
StatePublished - 5 Dec 2007
Externally publishedYes


  • CCR5 ligands
  • CD4 T lymphocytes
  • HIV
  • HIV envelope gp120
  • chemokines


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