Antibody responses to influenza A(H1N1)pdm infection

Steph Wraith; Raffael Nachbagauer; Angel Balmaseda; Daniel Stadlbauer; Sergio Ojeda; Arvind Rajabhathor; Roger Lopez; Andrea F. Guglia; Nery Sanchez; Fatima Amanat; Lionel Gresh; Guillermina Kuan; Florian Krammer; Aubree Gordon

doi:10.1016/j.vaccine.2020.04.069

Antibody responses to influenza A(H1N1)pdm infection

Steph Wraith, Raffael Nachbagauer, Angel Balmaseda, Daniel Stadlbauer, Sergio Ojeda, Arvind Rajabhathor, Roger Lopez, Andrea F. Guglia, Nery Sanchez, Fatima Amanat, Lionel Gresh, Guillermina Kuan, Florian Krammer, Aubree Gordon

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We investigated humoral immune response to influenza A(H1N1)pdm infection and found 32 (22%) of the infected individuals identified by PCR failed to produce a ≥ 4-fold hemagglutinin inhibition assay (HAI) response; a subset of 18 (56%) produced an alternate antibody response (against full-length HA, HA stalk, or neuraminidase). These individuals had lower pre-existing HAI antibody titers and showed a pattern of milder illness. An additional subset of 14 (44%) did not produce an alternate antibody response, had higher pre-existing antibody titers against full-length & stalk HA, and were less sick. These findings demonstrate that some individuals mount an alternate antibody response to influenza infection. In order to design more broadly protective influenza vaccines it may be useful to target these alternate sites. These findings support that there are influenza cases currently being missed by solely implementing HAI assays, resulting in an underestimation of the global burden of influenza infection.

Original language	English
Pages (from-to)	4221-4225
Number of pages	5
Journal	Vaccine
Volume	38
Issue number	27
DOIs	https://doi.org/10.1016/j.vaccine.2020.04.069
State	Published - 2 Jun 2020

Keywords

Antibodies
Hemagglutination inhibition
Influenza
Neuraminidase

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10.1016/j.vaccine.2020.04.069

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title = "Antibody responses to influenza A(H1N1)pdm infection",

abstract = "We investigated humoral immune response to influenza A(H1N1)pdm infection and found 32 (22%) of the infected individuals identified by PCR failed to produce a ≥ 4-fold hemagglutinin inhibition assay (HAI) response; a subset of 18 (56%) produced an alternate antibody response (against full-length HA, HA stalk, or neuraminidase). These individuals had lower pre-existing HAI antibody titers and showed a pattern of milder illness. An additional subset of 14 (44%) did not produce an alternate antibody response, had higher pre-existing antibody titers against full-length & stalk HA, and were less sick. These findings demonstrate that some individuals mount an alternate antibody response to influenza infection. In order to design more broadly protective influenza vaccines it may be useful to target these alternate sites. These findings support that there are influenza cases currently being missed by solely implementing HAI assays, resulting in an underestimation of the global burden of influenza infection.",

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AU - Wraith, Steph

AU - Nachbagauer, Raffael

AU - Balmaseda, Angel

AU - Stadlbauer, Daniel

AU - Ojeda, Sergio

AU - Rajabhathor, Arvind

AU - Lopez, Roger

AU - Guglia, Andrea F.

AU - Sanchez, Nery

AU - Amanat, Fatima

AU - Gresh, Lionel

AU - Kuan, Guillermina

AU - Krammer, Florian

AU - Gordon, Aubree

PY - 2020/6/2

Y1 - 2020/6/2

N2 - We investigated humoral immune response to influenza A(H1N1)pdm infection and found 32 (22%) of the infected individuals identified by PCR failed to produce a ≥ 4-fold hemagglutinin inhibition assay (HAI) response; a subset of 18 (56%) produced an alternate antibody response (against full-length HA, HA stalk, or neuraminidase). These individuals had lower pre-existing HAI antibody titers and showed a pattern of milder illness. An additional subset of 14 (44%) did not produce an alternate antibody response, had higher pre-existing antibody titers against full-length & stalk HA, and were less sick. These findings demonstrate that some individuals mount an alternate antibody response to influenza infection. In order to design more broadly protective influenza vaccines it may be useful to target these alternate sites. These findings support that there are influenza cases currently being missed by solely implementing HAI assays, resulting in an underestimation of the global burden of influenza infection.

AB - We investigated humoral immune response to influenza A(H1N1)pdm infection and found 32 (22%) of the infected individuals identified by PCR failed to produce a ≥ 4-fold hemagglutinin inhibition assay (HAI) response; a subset of 18 (56%) produced an alternate antibody response (against full-length HA, HA stalk, or neuraminidase). These individuals had lower pre-existing HAI antibody titers and showed a pattern of milder illness. An additional subset of 14 (44%) did not produce an alternate antibody response, had higher pre-existing antibody titers against full-length & stalk HA, and were less sick. These findings demonstrate that some individuals mount an alternate antibody response to influenza infection. In order to design more broadly protective influenza vaccines it may be useful to target these alternate sites. These findings support that there are influenza cases currently being missed by solely implementing HAI assays, resulting in an underestimation of the global burden of influenza infection.

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SP - 4221

EP - 4225

JO - Vaccine

JF - Vaccine

IS - 27

ER -

Antibody responses to influenza A(H1N1)pdm infection

Abstract

Keywords

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