Antibody response against poly (Glu60Ala30Tyr10) terpolymer and bacterial levan in ϰ‐deficient mice

Luminita Pricop, Akira Hatakeyama, Tom Moran, Constantin Bona

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    6 Scopus citations

    Abstract

    In murine species, the ϰ+‐bearing immunoglobulins‐dominate the antibody (Ab) repertoire with a ϰ/λ ratio of 95 : 5. The aim of the present study is to investigate the characteristics of the antibody response in ϰ‐deficient (K −/−) mice immunized with a T‐dependent synthetic antigen, poly (Glu60Ala30Tyr10) (GAT) and a T‐independent antigen, bacterial levan (BL). K −/− mice were obtained by targeted deletion of the JϰCϰ gene segments. In response to GAT, K −/− mice respond by producing increasing amounts of anti‐GAT Igλ1 and Igλ2 in the primary as well as secondary response, although anti‐GAT specific monoclonal antibodies (mAb) raised in K −/− mice are mostly of IgM isotype. The GAT public idiotype, GATIdX, present on all GAT‐specific Ab bearing x light chain, is not detected in the sera of K −/− mice or on any of the anti‐GATλ1 mAb. In response to BL, the amount of Igϰ1+ Ab in K −/− mice is comparable to the amount of Igϰ+ Ab in normal mice. However, λ2+ Ab are detected neither in wild‐type nor in K −/− mice. Like ϰ+ Ab, the majority of λ1+ mAb are specific for β2–6 fructosan present in BL and rye levan and, to some extent, express the BL‐specific idiotype, A48ld. Our results show that important compensatory mechanisms occur in ϰ‐deficient mice, restoring their ability to mount immune responses against a variety of T‐dependent and T‐independent antigens by the alternative usage of the clonally restricted λ repertoire.

    Original languageEnglish
    Pages (from-to)1039-1043
    Number of pages5
    JournalEuropean Journal of Immunology
    Volume25
    Issue number4
    DOIs
    StatePublished - Apr 1995

    Keywords

    • B cell repertoire
    • x chain
    • λ chain
    • ϰ‐deficient mice

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