TY - JOUR
T1 - Antibodies to the 70 kDa heat shock protein in midtrimester amniotic fluid and intraamniotic immunity
AU - Gelber, Shari E.
AU - Bongiovanni, Ann Marie
AU - Jean-Pierre, Claudel
AU - Linhares, Iara M.
AU - Skupski, Daniel W.
AU - Witkin, Steven S.
PY - 2007/9
Y1 - 2007/9
N2 - Objective: Antibodies to the 70 kDa heat shock protein (hsp70) immunoglobulin (Ig) G are markers for exposure to adverse or nonphysiological stimuli. In addition, these antibodies cross-link hsp-70 microbial antigen complexes and enhance development of antimicrobial immunity. The association between intraamniotic hsp70 IgG concentrations and intraamniotic immune responses were evaluated. Study Design: Midtrimester amniotic fluids from 90 women undergoing an amniocentesis were tested for hsp70 IgG, hsp70 antigen, tumor necrosis factor (TNF)-α, secretory leukocyte protease inhibitor (SLPI), and interferon (IFN)-α by enzyme-linked immunosorbent assay. Clinical outcomes were obtained after completion of all testing. Associations were analyzed by nonparametric statistics. Results: Intraamniotic hsp70 IgG concentrations, but not hsp70 antigen levels, were positively associated with levels of TNF-α (P < .0001), IFN-α (P = .0001), and SLPI (P = .0038). There were no associations between hsp70 IgG and maternal age or parity, race/ethnicity or pregnancy outcome. Conclusion: The hsp70 IgG levels correlate with intraamniotic concentrations of antimicrobial immune mediators. This antibody may potentiate antimicrobial immunity during fetal development.
AB - Objective: Antibodies to the 70 kDa heat shock protein (hsp70) immunoglobulin (Ig) G are markers for exposure to adverse or nonphysiological stimuli. In addition, these antibodies cross-link hsp-70 microbial antigen complexes and enhance development of antimicrobial immunity. The association between intraamniotic hsp70 IgG concentrations and intraamniotic immune responses were evaluated. Study Design: Midtrimester amniotic fluids from 90 women undergoing an amniocentesis were tested for hsp70 IgG, hsp70 antigen, tumor necrosis factor (TNF)-α, secretory leukocyte protease inhibitor (SLPI), and interferon (IFN)-α by enzyme-linked immunosorbent assay. Clinical outcomes were obtained after completion of all testing. Associations were analyzed by nonparametric statistics. Results: Intraamniotic hsp70 IgG concentrations, but not hsp70 antigen levels, were positively associated with levels of TNF-α (P < .0001), IFN-α (P = .0001), and SLPI (P = .0038). There were no associations between hsp70 IgG and maternal age or parity, race/ethnicity or pregnancy outcome. Conclusion: The hsp70 IgG levels correlate with intraamniotic concentrations of antimicrobial immune mediators. This antibody may potentiate antimicrobial immunity during fetal development.
KW - amniotic fluid
KW - heat shock protein
KW - heat shock protein antibodies
KW - immune regulation
KW - interferon-α
KW - secretory leukocyte protease inhibitor
KW - tumor necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=34548444214&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2007.06.019
DO - 10.1016/j.ajog.2007.06.019
M3 - Article
C2 - 17826419
AN - SCOPUS:34548444214
SN - 0002-9378
VL - 197
SP - 278.e1-278.e4
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 3
ER -