Antibodies recognizing CD24 LAP epitope on human T cells enhance CD28 and IL-2 T cell proliferation

María Del C. Salamone, Carolina Rosselot, Gabriela V. Salamone, Marcos Barboza, Miguel Kado, Leonardo Fainboim

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Membrane expression of the CD24 molecule on activated T lymphocytes is not elucidated fully. We previously described the intracellular and cell-surface expression of the CD24 sialic acid-dependent epitope(s) on phytohemagglutinin-activated peripheral blood mononuclear cells. However, the CD24 core protein was not detected previously on human T cells. This study reinvestigated the expression and role of CD24 in T cell subsets. We analyzed binding of anti-CD24 monoclonal antibodies (mAbs) to sialic and leucine-alanine-proline (LAP) epitopes in resting and activated, normal T lymphocytes. CD24 LAP and CD24 sialic epitopes were detected on activated CD4- and CD8-positive cells. Although expression of CD24 sialic epitopes remained stably expressed in interleukin (IL)-2-dependent cultures, T cell expression of the LAP epitope was transient. Anti-LAP antibodies strongly enhanced the response of T cells to a combination of anti-CD3/CD28 mAbs and enhanced proliferative response induced by recombinant IL-2. We found similarities in the tissue distribution and function of the human CD24 LAP molecule and the murine, heat-stable antigen, which suggests that CD24 might function as a signaling molecule on human T cells.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalJournal of Leukocyte Biology
Volume69
Issue number2
StatePublished - 2001
Externally publishedYes

Keywords

  • CD24 antigen
  • Costimulation signals
  • PBMC
  • Phytohemagglutinin

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