TY - JOUR
T1 - Antibodies elicited by SARS-CoV-2 infection or mRNA vaccines have reduced neutralizing activity against Beta and Omicron pseudoviruses
AU - Sievers, Benjamin L.
AU - Chakraborty, Saborni
AU - Xue, Yong
AU - Gelbart, Terri
AU - Gonzalez, Joseph C.
AU - Cassidy, Arianna G.
AU - Golan, Yarden
AU - Prahl, Mary
AU - Gaw, Stephanie L.
AU - Arunachalam, Prabhu S.
AU - Blish, Catherine A.
AU - Boyd, Scott D.
AU - Davis, Mark M.
AU - Jagannathan, Prasanna
AU - Nadeau, Kari C.
AU - Pulendran, Bali
AU - Singh, Upinder
AU - Scheuermann, Richard H.
AU - Frieman, Matthew B.
AU - Vashee, Sanjay
AU - Wang, Taia T.
AU - Tan, Gene S.
N1 - Publisher Copyright:
© 2022 The Authors.
PY - 2022/3/2
Y1 - 2022/3/2
N2 - Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that have mutations associated with increased transmission and antibody escape have arisen over the course of the current pandemic. Although the current vaccines have largely been effective against past variants, the number of mutations found on the Omicron (B.1.1.529) spike protein appear to diminish the protection conferred by preexisting immunity. Using vesicular stomatitis virus (VSV) pseudoparticles expressing the spike protein of several SARS-CoV-2 variants, we evaluated the magnitude and breadth of the neutralizing antibody response over time in individuals after infection and in mRNA-vaccinated individuals. We observed that boosting increases the magnitude of the antibody response to wild-type (D614), Beta, Delta, and Omicron variants; however, the Omicron variant was the most resistant to neutralization. We further observed that vaccinated healthy adults had robust and broad antibody responses, whereas responses may have been reduced in vaccinated pregnant women, underscoring the importance of learning how to maximize mRNA vaccine responses in pregnant populations. Findings from this study show substantial heterogeneity in the magnitude and breadth of responses after infection and mRNA vaccination and may support the addition of more conserved viral antigens to existing SARS-CoV-2 vaccines.
AB - Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants that have mutations associated with increased transmission and antibody escape have arisen over the course of the current pandemic. Although the current vaccines have largely been effective against past variants, the number of mutations found on the Omicron (B.1.1.529) spike protein appear to diminish the protection conferred by preexisting immunity. Using vesicular stomatitis virus (VSV) pseudoparticles expressing the spike protein of several SARS-CoV-2 variants, we evaluated the magnitude and breadth of the neutralizing antibody response over time in individuals after infection and in mRNA-vaccinated individuals. We observed that boosting increases the magnitude of the antibody response to wild-type (D614), Beta, Delta, and Omicron variants; however, the Omicron variant was the most resistant to neutralization. We further observed that vaccinated healthy adults had robust and broad antibody responses, whereas responses may have been reduced in vaccinated pregnant women, underscoring the importance of learning how to maximize mRNA vaccine responses in pregnant populations. Findings from this study show substantial heterogeneity in the magnitude and breadth of responses after infection and mRNA vaccination and may support the addition of more conserved viral antigens to existing SARS-CoV-2 vaccines.
UR - http://www.scopus.com/inward/record.url?scp=85123855842&partnerID=8YFLogxK
U2 - 10.1126/scitranslmed.abn7842
DO - 10.1126/scitranslmed.abn7842
M3 - Article
C2 - 35025672
AN - SCOPUS:85123855842
SN - 1946-6234
VL - 14
JO - Science Translational Medicine
JF - Science Translational Medicine
IS - 634
M1 - abn7842
ER -