Anti-VPg antibody inhibition of the poliovirus replicase reaction and production of covalent complexes of VPg-related proteins and RNA

Margaret H. Baron, David Baltimore

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Anti-VPg antibodies inhibited host-factor-dependent RNA synthesis by the poliovirus replicase but not oligo(U)-primed synthesis, implicating VPg in the de novo initiation of replicase products. Complexes of VPg-related polypeptide and newly made RNA could be immunoprecipitated by anti-VPg antibody from the host-factor-stimulated products of the replicase reaction. The complexes appeared to be covalently linked and involved 50 to 150 nucleotide chains of RNA that were RNAase-T1-resistant and could be largely poly(U).

Original languageEnglish
Pages (from-to)745-752
Number of pages8
JournalCell
Volume30
Issue number3
DOIs
StatePublished - Oct 1982
Externally publishedYes

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