Anti-neuraminidase and anti-HA stalk antibodies reduce the susceptibility to and infectivity of influenza A/H3N2 virus

  • Gregory Hoy
  • , Thomas Cortier
  • , Hannah E. Maier
  • , Guillermina Kuan
  • , Roger Lopez
  • , Nery Sanchez
  • , Sergio Ojeda
  • , Miguel Plazaola
  • , Daniel Stadlbauer
  • , Abigail Shotwell
  • , Angel Balmaseda
  • , Florian Krammer
  • , Simon Cauchemez
  • , Aubree Gordon

Research output: Contribution to journalArticlepeer-review

Abstract

Immune responses against neuraminidase (NA) and hemagglutinin (HA) are critical for developing effective influenza vaccines, yet their role in influenza transmission remains unclear. We conducted household transmission studies in Managua, Nicaragua, to assess the impact of anti-NA and anti-HA antibodies induced by natural infection on influenza A/H3N2 susceptibility and infectivity. Using mathematical models capturing household transmission dynamics, we found that high pre-existing antibody levels against the HA head (>31, [95% CrI 13–67]), HA stalk (>35, [95% CrI 11–66]), and NA (>31, [95% CrI 12–68]) are associated with reduced susceptibility to infection (relative susceptibility: HA head, 0.63 [95% CrI 0.42–0.98]; HA stalk, 0.66 [95% CrI 0.44–0.99]; NA, 0.49 [95% CrI 0.30–0.76]). HA stalk (>58 [95% CrI: 47–70]) and NA (>27 [95% CrI: 15–43]) are associated with reduced infectivity (relative infectivity: NA, 0.55 [95% CrI: 0.32–0.98], HA stalk 0.53 [95% CrI: 0.27–0.97]). These findings suggest that influenza vaccines designed to elicit NA immunity in addition to HA immunity may not only enhance protection against infection but also reduce onward transmission.

Original languageEnglish
Article number10910
JournalNature Communications
Volume16
Issue number1
DOIs
StatePublished - Dec 2025

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