Anti-inflammatory effects of Ganoderma lucidum triterpenoid in human Crohn's disease associated with downregulation of NF-κB signaling

Changda Liu, David Dunkin, Joanne Lai, Ying Song, Clare Ceballos, Keith Benkov, Xiu Min Li

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Background: Crohn's disease (CD) is a chronic inflammatory disease of the gastrointestinal tract. Current medications have potentially serious side effects. Hence, there is increasing interest in alternative therapies. We previously demonstrated the anti-inflammatory effects of Food Allergy Herbal Formula-2 in vitro on peripheral blood mononuclear cells (PBMCs) and mucosa from CD subjects. Here, we investigated the anti-inflammatory effects of a bioactive compound isolated from Ganoderma lucidum (G. lucidum), a key herbal constituent of Food Allergy Herbal Formula-2, in CD in vitro. Methods: Triterpene ganoderic acid C1 (GAC1) was isolated from G. lucidum. Stimulated RAW 264.7 macrophages were treated with GAC1. Human PBMCs and colonic biopsies were obtained from children with CD and cultured with or without GAC1. TNF-α and other proinflammatory cytokine levels were measured in the culture supernatant. NF-κB signaling was investigated in PBMCs and colonic mucosa treated with GAC1 by In-Cell Western and Western blot analysis. Results: GAC1 decreased TNF-α production by macrophages and PBMCs from CD subjects. GAC1 significantly decreased TNF-α, IFN-γ, and IL-17A production by inflamed colonic biopsies from CD subjects. These effects were due to downregulation of the NF-κB signaling pathway. Conclusions: GAC1 inhibited production of TNF-α and other proinflammatory cytokines by PBMCs and inflamed CD colonic mucosa due to blockage of NF-κB activation. GAC1 is a key beneficial constituent in G. lucidum and the Food Allergy Herbal Formula-2 in suppressing the inflammatory cytokines found in CD and warrants clinical investigation for the treatment of CD.

Original languageEnglish
Pages (from-to)1918-1925
Number of pages8
JournalInflammatory Bowel Diseases
Volume21
Issue number8
DOIs
StatePublished - 29 Apr 2015

Keywords

  • Crohn's disease
  • FAHF-2
  • NF-κB
  • TNF-α
  • Triterpene

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