Anti-inflammation effects of highly purified low-Mw RG-I pectins on LPS-activated macrophages

Dongmei Wu, Xiaoliang Zheng, Weiwei Hu, Kai Zhu, Chengxiao Yu, Qiaojun He, Robert J. Linhardt, Xingqian Ye, Shiguo Chen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Pectic oligosaccharides are viewed as excellent immunomodulator, but the specific molecular weight (Mw) range required for anti-inflammatory effects remains unclear. In this study, citrus pectins was depolymerized by the ultrasound/H2O2/Vc system and the pectic mixtures was separated through the superdex 30 chromatography column. Three highly purified pectin fractions (USP1, USP2, USP3) were obtained, with the Mw of 2713 g/mol, 3683 g/mol, and 7469 g/mol. All of them were RG-I pectins and USP3 contained more arabinan sidechains than USP1 and USP2. In vitro anti-inflammation assays based on LPS-induced RAW264.7 macrophages indicated that these pectins could suppress the NO release, ROS production and down-regulated the level of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in a Mw-dependent manner. The inhibitory effects increased in the order of USP1<USP2<USP3. Meanwhile, the regulation of pro-inflammatory cytokines were closely related to the content of arabinan sidechains in pectin. Thus, low-Mw pectins with Mw>3KD should be effective as a prophylactic agent against inflammatory diseases in functional food.

Original languageEnglish
Article number100283
JournalBioactive Carbohydrates and Dietary Fibre
Volume26
DOIs
StatePublished - Nov 2021
Externally publishedYes

Keywords

  • Anti-inflammatory
  • Arabinan
  • IL-6
  • Molecular weight
  • Pro-inflammatory cytokines
  • RG-I pectin
  • Sidechains

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