Angiotensin II stimulates basolateral K channels in rat cortical collecting ducts

Yuan Wei, Wenhui Wang

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We used the patch-clamp technique to study the effects of angiotensin II (ANG II) on basolateral K channels in cortical collecting ducts (CCDs). Application of ANG II (100 pM-100 nM) increased the activity of basolateral 18-pS K channels. This effect of ANG II was completely abolished by losartan, which is an antagonist of type 1 angiotensin (AT1) receptors. In contrast, inhibition of type 2 angiotensin (AT2) receptors did not block the stimulatory effect of ANG II. Also, application of ANG II significantly increased intracellular Ca2+ concentrations, which were measured with fura 2 dye. To explore the role of Ca2+-dependent pathways in the regulation of basolateral K channels, the effects of ANG II on channel activity were examined in the presence of arachidonyltrifluoromethyl ketone to inhibit phospholipase A2 (PLA2), GF-109203X [a protein kinase C (PKC) inhibitor], and NG-nitro-L-arginine methyl ester (L-NAME) to inhibit nitric oxide synthase. Inhibition of either PLA2 or PKC did not block the effect of ANG II on basolateral K-channel activity. However, the stimulatory effect of ANG II was absent in the CCDs treated with L-NAME. Moreover, addition of the membrane-permeant 8-bromo-guanosine 3′,5′-cyclic monophosphate (8-bromo-cGMP) not only increased channel activity but also abolished the stimulatory effect of ANG II on channel activity. We conclude that ANG II increases basolateral K-channel activity via the stimulation of AT1 receptors, and the stimulatory effect of ANG II is mediated by a nitric oxide-dependent cGMP pathway.

Original languageEnglish
Pages (from-to)F175-F181
JournalAmerican Journal of Physiology - Renal Physiology
Volume284
Issue number1 53-1
DOIs
StatePublished - 1 Jan 2003
Externally publishedYes

Keywords

  • Angiotensin type 1 receptor
  • Guanosine 3′,5′-cyclic monophosphate
  • Nitric oxide
  • Potassium secretion

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