Angiogenic profile of childhood primitive neuroectodermal brain tumours/medulloblastomas

H. Huber, A. Eggert, A. J. Janss, R. Wiewrodt, H. Zhao, L. N. Sutton, L. B. Rorke, P. C. Phillips, M. A. Grotzer

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Primitive neuroectodermal brain tumours (PNET) including medulloblastomas (PNET/MB) are the most common malignant brain tumours of childhood. Similar to many other brain tumours, PNET/MB often show marked neovascularisation. To determine which angiogenic factors contribute to PNET/MB angiogenesis, we examined the expression of eight angiogenic factors (vascular endothelial growth factors (VEGF, VEGF-B, VEGF-C), basic fibroblast growth factor (bFGF), angiopoetins (Ang-1, Ang-2), transforming growth factor (TGF-α), and platelet-derived endothelial growth factor (PDGF-A)) by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in six PNET cell lines and 28 primary PNET/MB. Expression levels of angiogenic factors were compared with microvessel density, TrkC mRNA expression, clinical variables and survival outcomes. Our results indicate that all PNET/MB tested produce a wide range of angiogenic factors that are, individually or together, likely to play a direct role in PNET/MB tumour growth. This suggests that anti-angiogenesis approaches targeting VEGF alone may be insufficient in PNET/MB.

Original languageEnglish
Pages (from-to)2064-2072
Number of pages9
JournalEuropean Journal of Cancer
Volume37
Issue number16
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Angiogenesis
  • Angiopoetin
  • Basic fibroblast growth factor
  • Brain tumour
  • Medulloblastoma
  • Platelet-derived endothelial growth factor
  • Transforming growth factor-α
  • Vascular endothelial growth factor

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