Abstract
Although androgens are believed to influence the distribution of human adipose tissue and have been detected in human fat, receptors for these sex hormones have yet to be identified. These studies demonstrate that a high-affinity, limited-capacity binding component for the synthetic androgen methyltrienolone (R1881) exists in ammonium sulfate precipitates of human adipose tissue cytosols. The equilibrium dissociation constant (Kd = 0.1 to 0.4 nmoll L, n = 6) and the number of binding sites (2 to 26 fmollmg protein, n = 22) are consistent with those reported for androgen receptors in rat prostate, human prostatic carcinoma, MCF-7 cells, and baboon myocardium. The relative steroidinding specificities of the human adipose tissue androphile (R1881~5α-ihydrotestosterone>testosterone> estradiol~progesterone>dexamethasone) are similar, but not identical, to those reported for androgen receptors in rat prostate (Rl88l>5α-dihydrotestosterone~testosterone>estradiol>progesterone> cortisol) and baboon myocardium (R1881>5α-dihydrotestosterone.testosterone>progesterone> estradiol>cortisol). The function of the androgen-binding component in human adipose tissue is not known. (Steroids 55:410-415, 1990).
| Original language | English |
|---|---|
| Pages (from-to) | 410-415 |
| Number of pages | 6 |
| Journal | Steroids |
| Volume | 55 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1990 |
| Externally published | Yes |
Keywords
- androgen binding
- human adipose tissue
- methyltrienolone binding
- steroid binding
- steroids