Analysis of viral DNA in isolates from patients with recurrent herpetic keratitis

P. A. Asbell; Y. M. Centifanto-Fitzgerald; J. W. Chandler; H. E. Kaufman

Analysis of viral DNA in isolates from patients with recurrent herpetic keratitis

P. A. Asbell
, Y. M. Centifanto-Fitzgerald
, J. W. Chandler
, H. E. Kaufman

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Isolates were obtained from 10 patients with recurrent herpes simplex type 1 infection of the eye, lids, or mouth. The viral DNA of successive isolates from each patient was analyzed by restriction endonuclease fingerprinting. Evaluation of the DNA banding patterns of all isolates by means of two different enzymes, performed in a masked fashion, demonstrated that all of the isolates from any one patient had the same genetic makeup. These results indicate that recurrent herpes infections of the eye and face in humans are caused not by unrelated, serial infections but rather by reactivation of the same latent virus that remains in the ganglion over a period of years.

Original language	English
Pages (from-to)	951-954
Number of pages	4
Journal	Investigative Ophthalmology and Visual Science
Volume	25
Issue number	8
State	Published - 1984
Externally published	Yes

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title = "Analysis of viral DNA in isolates from patients with recurrent herpetic keratitis",

abstract = "Isolates were obtained from 10 patients with recurrent herpes simplex type 1 infection of the eye, lids, or mouth. The viral DNA of successive isolates from each patient was analyzed by restriction endonuclease fingerprinting. Evaluation of the DNA banding patterns of all isolates by means of two different enzymes, performed in a masked fashion, demonstrated that all of the isolates from any one patient had the same genetic makeup. These results indicate that recurrent herpes infections of the eye and face in humans are caused not by unrelated, serial infections but rather by reactivation of the same latent virus that remains in the ganglion over a period of years.",

author = "Asbell, \{P. A.\} and Centifanto-Fitzgerald, \{Y. M.\} and Chandler, \{J. W.\} and Kaufman, \{H. E.\}",

year = "1984",

language = "English",

volume = "25",

pages = "951--954",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

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T1 - Analysis of viral DNA in isolates from patients with recurrent herpetic keratitis

AU - Asbell, P. A.

AU - Centifanto-Fitzgerald, Y. M.

AU - Chandler, J. W.

AU - Kaufman, H. E.

PY - 1984

Y1 - 1984

N2 - Isolates were obtained from 10 patients with recurrent herpes simplex type 1 infection of the eye, lids, or mouth. The viral DNA of successive isolates from each patient was analyzed by restriction endonuclease fingerprinting. Evaluation of the DNA banding patterns of all isolates by means of two different enzymes, performed in a masked fashion, demonstrated that all of the isolates from any one patient had the same genetic makeup. These results indicate that recurrent herpes infections of the eye and face in humans are caused not by unrelated, serial infections but rather by reactivation of the same latent virus that remains in the ganglion over a period of years.

AB - Isolates were obtained from 10 patients with recurrent herpes simplex type 1 infection of the eye, lids, or mouth. The viral DNA of successive isolates from each patient was analyzed by restriction endonuclease fingerprinting. Evaluation of the DNA banding patterns of all isolates by means of two different enzymes, performed in a masked fashion, demonstrated that all of the isolates from any one patient had the same genetic makeup. These results indicate that recurrent herpes infections of the eye and face in humans are caused not by unrelated, serial infections but rather by reactivation of the same latent virus that remains in the ganglion over a period of years.

UR - https://www.scopus.com/pages/publications/0021150890

M3 - Article

C2 - 6086547

AN - SCOPUS:0021150890

SN - 0146-0404

VL - 25

SP - 951

EP - 954

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

IS - 8

ER -

Analysis of viral DNA in isolates from patients with recurrent herpetic keratitis

Abstract

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