TY - JOUR
T1 - Analysis of ultraviolet-induced RNA-RNA cross-links
T2 - A means for probing RNA structure-function relationships
AU - Branch, Andrea D.
AU - Benenfeld, Bonnie J.
AU - Paul, Cynthia P.
AU - Robertson, Hugh D.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - This chapter presents analysis of ultraviolet-induced RNA–RNA cross-links. To obtain maximum information about local bonding patterns, the UV-induced cross-link must be mapped to the nucleotide level. Direct RNA analysis is needed. However, if cross-links are to be used only to determine which portions of the RNA are close together in the three-dimensional structure, indirect techniques, such as primer extension, may be employed. The mapping of an extensive series of RNA–RNA cross-links introduced into rRNA has led to a detailed three-dimensional picture of 16 S rRNA. While additional procedures are usually required to locate a UV-induced bond in a large RNA molecule, a cross-link in an RNA smaller than 1000 nt can often be detected directly by a change the cross-link makes in the characteristic pattern of ribonuclease-resistant oligonucleotides. Primer extension is useful for mapping cross-links, especially when employed in combination with direct RNA analysis.
AB - This chapter presents analysis of ultraviolet-induced RNA–RNA cross-links. To obtain maximum information about local bonding patterns, the UV-induced cross-link must be mapped to the nucleotide level. Direct RNA analysis is needed. However, if cross-links are to be used only to determine which portions of the RNA are close together in the three-dimensional structure, indirect techniques, such as primer extension, may be employed. The mapping of an extensive series of RNA–RNA cross-links introduced into rRNA has led to a detailed three-dimensional picture of 16 S rRNA. While additional procedures are usually required to locate a UV-induced bond in a large RNA molecule, a cross-link in an RNA smaller than 1000 nt can often be detected directly by a change the cross-link makes in the characteristic pattern of ribonuclease-resistant oligonucleotides. Primer extension is useful for mapping cross-links, especially when employed in combination with direct RNA analysis.
UR - http://www.scopus.com/inward/record.url?scp=0024847292&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(89)80115-6
DO - 10.1016/0076-6879(89)80115-6
M3 - Article
C2 - 2482427
AN - SCOPUS:0024847292
SN - 0076-6879
VL - 180
SP - 418
EP - 442
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -