Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice

Andrei M. Vacaru; Joan Isern; Stuart T. Fraser; Margaret H. Baron

doi:10.1002/dvg.22420

Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice

Andrei M. Vacaru, Joan Isern, Stuart T. Fraser, Margaret H. Baron

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage. genesis 51:751-762.

Original language	English
Pages (from-to)	751-762
Number of pages	12
Journal	Genesis
Volume	51
Issue number	11
DOIs	https://doi.org/10.1002/dvg.22420
State	Published - Nov 2013

Keywords

Cyan fluorescent protein
Fluorescent reporter
Histone fusion
Imaging
Primitive erythropoiesis
Transgenic mice

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10.1002/dvg.22420

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title = "Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice",

abstract = "Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage. genesis 51:751-762.",

keywords = "Cyan fluorescent protein, Fluorescent reporter, Histone fusion, Imaging, Primitive erythropoiesis, Transgenic mice",

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year = "2013",

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doi = "10.1002/dvg.22420",

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T1 - Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice

AU - Vacaru, Andrei M.

AU - Isern, Joan

AU - Fraser, Stuart T.

AU - Baron, Margaret H.

PY - 2013/11

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AB - Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage. genesis 51:751-762.

KW - Cyan fluorescent protein

KW - Fluorescent reporter

KW - Histone fusion

KW - Imaging

KW - Primitive erythropoiesis

KW - Transgenic mice

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Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice

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Keywords

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