Analysis of intracellular PTEN signaling and secretion

Cindy Hodakoski, Barry Fine, Benjamin Hopkins, Ramon Parsons

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The tumor suppressor PTEN dephosphorylates PIP3 to inhibit PI3K signaling in cells. Altering PTEN intracellular signaling can therefore significantly affect cell behavior. Two novel mechanisms of PTEN regulation including the secretion and entry of the translational variant PTEN-L, and enzymatic inhibition by the interacting protein P-REX2, have been shown to modulate PI3K signaling, cellular proliferation and survival, and glucose metabolism. Here, we review the methods used to identify and validate the existence of both PTEN-L and the P-REX2-PTEN complex, to determine their effects on PTEN phosphatase activity, and to examine their role in cellular physiology.

Original languageEnglish
Pages (from-to)164-171
Number of pages8
StatePublished - 1 May 2015


  • P-REX2
  • PTEN
  • PTEN-L
  • Secretion


Dive into the research topics of 'Analysis of intracellular PTEN signaling and secretion'. Together they form a unique fingerprint.

Cite this