Analysis of human blood memory T follicular helper subsets

Salah Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, Hideki Ueno

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Human blood contains a memory counterpart of T follicular helper (TFH) cells. Blood TFH cells are composed of subsets that differ in phenotype and function. Recent studies show that analysis of blood circulating memory TFH cells can provide clues to understand the mode of actions of vaccines and the pathogenesis of human autoimmune diseases. We will describe here a detailed protocol to analyze the memory TFH subsets in human whole blood samples. We will also describe a protocol to assess the helper capacity of blood memory TFH subsets.

Original languageEnglish
Pages (from-to)187-197
Number of pages11
JournalMethods in Molecular Biology
Volume1291
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • Blood
  • CXCR5
  • Human
  • Subsets
  • T follicular helper cells

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