Analysis of Glycosaminoglycans in Stem Cell Glycomics

Boyangzi Li, Haiying Liu, Zhenqing Zhang, Hope E. Stansfield, Jonathan S. Dordick, Robert J. Linhardt

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Scopus citations

Abstract

Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

Original languageEnglish
Title of host publicationEmbryonic Stem Cell Therapy for Osteo-Degenerative Diseases
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages285-300
Number of pages16
ISBN (Print)9781607619611
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume690
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chondroitin sulfate
  • Dermatan sulfate
  • Disaccharide analysis
  • Enzymatic digestion
  • Glycosaminoglycans
  • Heparan sulfate
  • Heparin
  • LC-MS
  • NCCIT cells
  • Purification

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