Analysis of genetic and epigenetic processes that influence p14ARF expression in breast cancer

Javier Silva, Gemma Domínguez, José M. Silva, Jose M. García, Isabel Gallego, César Corbacho, Mariano Provencio, Pilar Espãa, Félix Bonilla

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


The INK4a/ARF locus encodes two unrelated cell cycle-regulatory proteins that both function in tumor suppression, p16INK4a and p14ARF. In human tumors including breast cancer, alterations affecting selectively p14ARF have been poorly analysed. We have performed a comprehensive analysis of the inactivation mechanisms (mutation, homozygous and hemizygous deletion, and promoter hypermethylation) in a large series of 100 primary breast carcinomas. RT-PCR showed expression variable of the p14ARF transcript, with 17% demonstrating overexpression and 26% demonstrating decreased expression. No detectable alterations were observed in the majority of cases with overexpressed p14ARF mRNA, but 77% of tumors with decreased expression presented at least one of these genetic/epigenetic alterations. Nevertheless, a statistically significant correlation was observed between decreased p14ARF expression and several poor prognostic parameters.

Original languageEnglish
Pages (from-to)4586-4590
Number of pages5
Issue number33
StatePublished - 27 Jul 2001
Externally publishedYes


  • Allelic losses
  • Breast cancer
  • Hypermethylation
  • Mutations
  • Overexpression
  • p14ARF


Dive into the research topics of 'Analysis of genetic and epigenetic processes that influence p14ARF expression in breast cancer'. Together they form a unique fingerprint.

Cite this