Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry

Michael W. Wolff; Hélène G. Bazin; Robert J. Lindhardt

doi:10.1023/A:1008961114201

Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry

Michael W. Wolff, Hélène G. Bazin, Robert J. Lindhardt

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.

Original language	English
Pages (from-to)	797-801
Number of pages	5
Journal	Biotechnology Techniques
Volume	13
Issue number	11
DOIs	https://doi.org/10.1023/A:1008961114201
State	Published - 1999
Externally published	Yes

Keywords

Electrospray mass spectrometry
Exoglycosidase
Neutral oligosaccharide
Preparative capillary electrophoresis

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title = "Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry",

abstract = "Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.",

keywords = "Electrospray mass spectrometry, Exoglycosidase, Neutral oligosaccharide, Preparative capillary electrophoresis",

author = "Wolff, \{Michael W.\} and Bazin, \{H{\'e}l{\`e}ne G.\} and Lindhardt, \{Robert J.\}",

year = "1999",

doi = "10.1023/A:1008961114201",

language = "English",

volume = "13",

pages = "797--801",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Springer Science and Business Media B.V.",

number = "11",

}

TY - JOUR

T1 - Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry

AU - Wolff, Michael W.

AU - Bazin, Hélène G.

AU - Lindhardt, Robert J.

PY - 1999

Y1 - 1999

N2 - Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.

AB - Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed.

KW - Electrospray mass spectrometry

KW - Exoglycosidase

KW - Neutral oligosaccharide

KW - Preparative capillary electrophoresis

UR - https://www.scopus.com/pages/publications/0032795863

U2 - 10.1023/A:1008961114201

DO - 10.1023/A:1008961114201

M3 - Article

AN - SCOPUS:0032795863

SN - 0951-208X

VL - 13

SP - 797

EP - 801

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 11

ER -

Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry

Abstract

Keywords

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