TY - JOUR
T1 - Analysis of cDNA and genomic clones coding for the proα1 chain of calf type II collagen
AU - Sangiorgi, Frank O.
AU - Benson-Chanda, Virginia
AU - de Wet, Wouter J.
AU - Sobel, Mark E.
AU - Ramirez, Francesco
N1 - Funding Information:
ACKNOWLEDGEMENTS We wish to thank Dr. Capon for the kind gift of the bovine genomic library and our colleagues Drs. M.-L. Chu, Y. Ninomiya, B. Olsen, D. Weil and M. Young for many helpful discussions and suggestions. We also gratefully acknowledge the invaluable help of Messrs. M. Bernard and D. Woodbury for the analysis of the sequencing data and Ms. L. Bertha and Ms. K. Valentine for typing the manuscript. This work was supported by grants from the National Institutes of
PY - 1985/4/25
Y1 - 1985/4/25
N2 - A bovine cDNA library constructed from fetal cartilage RNA was screened with a proα1(II) collagen specific chicken cDNA. A recombinant clone (Bc 7), with an insert of 1 kb, was identified and shown to contain sequences exhibiting 85% homology with the chicken proaα1(II) collagen C-propeptide. Interspecies comparison strongly suggested that one potential glycosylation site present in the avian C-propeptide is not utilized, since this site is absent in the bovine chain. In addition, two overlapping genomic clones (Pal 3 and Pal 4) were isolated and partially characterized. These clones span 23 kb of DNA and contain approximately 17 kb of the proα1(II) calf gene. Sequencing of exon 1 has determined the length of the 3′ untranslated region and the exact location of the polyadenylation attachment site.
AB - A bovine cDNA library constructed from fetal cartilage RNA was screened with a proα1(II) collagen specific chicken cDNA. A recombinant clone (Bc 7), with an insert of 1 kb, was identified and shown to contain sequences exhibiting 85% homology with the chicken proaα1(II) collagen C-propeptide. Interspecies comparison strongly suggested that one potential glycosylation site present in the avian C-propeptide is not utilized, since this site is absent in the bovine chain. In addition, two overlapping genomic clones (Pal 3 and Pal 4) were isolated and partially characterized. These clones span 23 kb of DNA and contain approximately 17 kb of the proα1(II) calf gene. Sequencing of exon 1 has determined the length of the 3′ untranslated region and the exact location of the polyadenylation attachment site.
UR - http://www.scopus.com/inward/record.url?scp=0022432041&partnerID=8YFLogxK
U2 - 10.1093/nar/13.8.2815
DO - 10.1093/nar/13.8.2815
M3 - Article
C2 - 2582365
AN - SCOPUS:0022432041
SN - 0305-1048
VL - 13
SP - 2815
EP - 2826
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 8
ER -