Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro

M. Longo; F. Fiorito; G. Marfè; S. Montagnaro; G. Pisanelli; L. De Martino; G. Iovane; U. Pagnini

doi:10.1016/j.virusres.2009.07.008

Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro

M. Longo, F. Fiorito, G. Marfè, S. Montagnaro, G. Pisanelli, L. De Martino, G. Iovane, U. Pagnini

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a "cross-talk" between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitro.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Virus Research
Volume	145
Issue number	2
DOIs	https://doi.org/10.1016/j.virusres.2009.07.008
State	Published - Nov 2009
Externally published	Yes

Keywords

Apoptosis
Bcl-2 family
Caspases
CpHV-1
Cytochrome c
PARP

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10.1016/j.virusres.2009.07.008

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title = "Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro",

abstract = "It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a {"}cross-talk{"} between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitro.",

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author = "M. Longo and F. Fiorito and G. Marf{\`e} and S. Montagnaro and G. Pisanelli and {De Martino}, L. and G. Iovane and U. Pagnini",

year = "2009",

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AU - Longo, M.

AU - Fiorito, F.

AU - Marfè, G.

AU - Montagnaro, S.

AU - Pisanelli, G.

AU - De Martino, L.

AU - Iovane, G.

AU - Pagnini, U.

PY - 2009/11

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N2 - It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a "cross-talk" between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitro.

AB - It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a "cross-talk" between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitro.

KW - Apoptosis

KW - Bcl-2 family

KW - Caspases

KW - CpHV-1

KW - Cytochrome c

KW - PARP

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VL - 145

SP - 227

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JO - Virus Research

JF - Virus Research

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ER -

Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro

Abstract

Keywords

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