Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts

Michael Bomble; Frank Tacke; Lothar Rink; Evgenia Kovalenko; Ralf Weiskirchen

doi:10.1016/j.bbrc.2010.04.094

Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts

Michael Bomble, Frank Tacke, Lothar Rink, Evgenia Kovalenko, Ralf Weiskirchen

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28 Scopus citations

Abstract

Here, we demonstrate that hepatic stellate cells (HSC) isolated from Lewis rats have in vitro antigen-presentation cell (APC) functionality and are able to process and present exogenous antigens. We show activation of a major histocompatibility complex II (RT1BI)-restricted T-cell hybridoma specific for guinea pig myelin basic protein (gpMBP) after coculture with HSC. During transdifferentiation of HSC into myofibroblasts (MFB) the APC function was markedly decreased but restorable by addition of interferon-γ (IFN-γ). Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-γ treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB.

Original language	English
Pages (from-to)	342-347
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	396
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.04.094
State	Published - 28 May 2010
Externally published	Yes

Keywords

Antigen presentation
Co-culture systems
Hepatic stellate cell
Immune regulation
Interferon-γ
Myofibroblast
T-cell response
Transdifferentiation

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10.1016/j.bbrc.2010.04.094

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title = "Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts",

abstract = "Here, we demonstrate that hepatic stellate cells (HSC) isolated from Lewis rats have in vitro antigen-presentation cell (APC) functionality and are able to process and present exogenous antigens. We show activation of a major histocompatibility complex II (RT1BI)-restricted T-cell hybridoma specific for guinea pig myelin basic protein (gpMBP) after coculture with HSC. During transdifferentiation of HSC into myofibroblasts (MFB) the APC function was markedly decreased but restorable by addition of interferon-γ (IFN-γ). Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-γ treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB.",

keywords = "Antigen presentation, Co-culture systems, Hepatic stellate cell, Immune regulation, Interferon-γ, Myofibroblast, T-cell response, Transdifferentiation",

author = "Michael Bomble and Frank Tacke and Lothar Rink and Evgenia Kovalenko and Ralf Weiskirchen",

note = "Funding Information: We thank Thomas Herrmann, University of W{\"u}rzburg for providing the cell lines 53/4 and L929-RT1BI and for comments on the manuscript. This work was supported by the START program of the Faculty of Medicine, RWTH-University Hospital Aachen to RW. ",

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language = "English",

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AU - Bomble, Michael

AU - Tacke, Frank

AU - Rink, Lothar

AU - Kovalenko, Evgenia

AU - Weiskirchen, Ralf

N1 - Funding Information: We thank Thomas Herrmann, University of Würzburg for providing the cell lines 53/4 and L929-RT1BI and for comments on the manuscript. This work was supported by the START program of the Faculty of Medicine, RWTH-University Hospital Aachen to RW.

PY - 2010/5/28

Y1 - 2010/5/28

N2 - Here, we demonstrate that hepatic stellate cells (HSC) isolated from Lewis rats have in vitro antigen-presentation cell (APC) functionality and are able to process and present exogenous antigens. We show activation of a major histocompatibility complex II (RT1BI)-restricted T-cell hybridoma specific for guinea pig myelin basic protein (gpMBP) after coculture with HSC. During transdifferentiation of HSC into myofibroblasts (MFB) the APC function was markedly decreased but restorable by addition of interferon-γ (IFN-γ). Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-γ treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB.

AB - Here, we demonstrate that hepatic stellate cells (HSC) isolated from Lewis rats have in vitro antigen-presentation cell (APC) functionality and are able to process and present exogenous antigens. We show activation of a major histocompatibility complex II (RT1BI)-restricted T-cell hybridoma specific for guinea pig myelin basic protein (gpMBP) after coculture with HSC. During transdifferentiation of HSC into myofibroblasts (MFB) the APC function was markedly decreased but restorable by addition of interferon-γ (IFN-γ). Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-γ treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB.

KW - Antigen presentation

KW - Co-culture systems

KW - Hepatic stellate cell

KW - Immune regulation

KW - Interferon-γ

KW - Myofibroblast

KW - T-cell response

KW - Transdifferentiation

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DO - 10.1016/j.bbrc.2010.04.094

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AN - SCOPUS:77952742518

SN - 0006-291X

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SP - 342

EP - 347

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts

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Keywords

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