Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia

Bianjiang Liu; Peng Wang; Zengjun Wang; Yuejun Jia; Xiaobing Niu; Wei Wang; Wei Zhang

doi:10.1007/s10815-010-9466-8

Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia

Bianjiang Liu, Peng Wang, Zengjun Wang, Yuejun Jia, Xiaobing Niu, Wei Wang, Wei Zhang

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Purpose: To analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia. Methods: High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility. Results: Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups. Conclusion: The high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.

Original language	English
Pages (from-to)	719-724
Number of pages	6
Journal	Journal of Assisted Reproduction and Genetics
Volume	27
Issue number	12
DOIs	https://doi.org/10.1007/s10815-010-9466-8
State	Published - Dec 2010
Externally published	Yes

Keywords

Asthenozoospermia
Spermatozoa
Voltage-dependent anion channel
mRNA

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10.1007/s10815-010-9466-8

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@article{ba99b7a0bcc145e2a64c02eab9114487,

title = "Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia",

abstract = "Purpose: To analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia. Methods: High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility. Results: Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups. Conclusion: The high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.",

keywords = "Asthenozoospermia, Spermatozoa, Voltage-dependent anion channel, mRNA",

author = "Bianjiang Liu and Peng Wang and Zengjun Wang and Yuejun Jia and Xiaobing Niu and Wei Wang and Wei Zhang",

note = "Funding Information: Acknowledgments We thank Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University for the technical assistance. This work was supported by the grant from National Natural Science Foundation of China (30872575).",

year = "2010",

month = dec,

doi = "10.1007/s10815-010-9466-8",

language = "English",

volume = "27",

pages = "719--724",

journal = "Journal of Assisted Reproduction and Genetics",

issn = "1058-0468",

publisher = "Springer",

number = "12",

}

Liu, B, Wang, P, Wang, Z, Jia, Y, Niu, X, Wang, W & Zhang, W 2010, 'Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia', Journal of Assisted Reproduction and Genetics, vol. 27, no. 12, pp. 719-724. https://doi.org/10.1007/s10815-010-9466-8

Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia. / Liu, Bianjiang; Wang, Peng; Wang, Zengjun et al.
In: Journal of Assisted Reproduction and Genetics, Vol. 27, No. 12, 12.2010, p. 719-724.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia

AU - Liu, Bianjiang

AU - Wang, Peng

AU - Wang, Zengjun

AU - Jia, Yuejun

AU - Niu, Xiaobing

AU - Wang, Wei

AU - Zhang, Wei

N1 - Funding Information: Acknowledgments We thank Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University for the technical assistance. This work was supported by the grant from National Natural Science Foundation of China (30872575).

PY - 2010/12

Y1 - 2010/12

N2 - Purpose: To analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia. Methods: High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility. Results: Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups. Conclusion: The high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.

AB - Purpose: To analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia. Methods: High motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility. Results: Real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups. Conclusion: The high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.

KW - Asthenozoospermia

KW - Spermatozoa

KW - Voltage-dependent anion channel

KW - mRNA

UR - https://www.scopus.com/pages/publications/78651326754

U2 - 10.1007/s10815-010-9466-8

DO - 10.1007/s10815-010-9466-8

M3 - Article

C2 - 20809416

AN - SCOPUS:78651326754

SN - 1058-0468

VL - 27

SP - 719

EP - 724

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

IS - 12

ER -

Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia

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Keywords

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