An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains

Claudia Link; Thomas Ebensen; Lothar Ständner; Marion Déjosez; Elena Reinhard; Faiza Rharbaoui; Carlos A. Guzmán

doi:10.1016/j.micinf.2006.04.013

An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains

Claudia Link, Thomas Ebensen, Lothar Ständner, Marion Déjosez, Elena Reinhard, Faiza Rharbaoui, Carlos A. Guzmán

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Salmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivatives. Cells infected with the double sseC sopB, phoP sopB and aroA sopB mutants also exhibited higher expression of MHC, CD80, CD86 and CD54 molecules, and showed a stronger capacity to process and present an I-E^d-restricted epitope from the influenza hemagglutinin (HA) to CD4⁺ cells from TCR-HA transgenic mice in vitro. The incorporation of an additional mutation into the sopB locus of the attenuated sseC, phoP and aroA mutants resulted in the stimulation of improved humoral and cellular immune responses following oral vaccination. The obtained results define a new potential immune escape strategy of this important pathogen, and also demonstrate that this mechanism can be subverted to optimize the immune responses elicited using Salmonella as a live vaccine carrier.

Original language	English
Pages (from-to)	2262-2269
Number of pages	8
Journal	Microbes and Infection
Volume	8
Issue number	8
DOIs	https://doi.org/10.1016/j.micinf.2006.04.013
State	Published - Jul 2006
Externally published	Yes

Keywords

Antigen presentation
Bacterial infection
Dendritic cells
Vaccination

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title = "An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains",

abstract = "Salmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivatives. Cells infected with the double sseC sopB, phoP sopB and aroA sopB mutants also exhibited higher expression of MHC, CD80, CD86 and CD54 molecules, and showed a stronger capacity to process and present an I-Ed-restricted epitope from the influenza hemagglutinin (HA) to CD4+ cells from TCR-HA transgenic mice in vitro. The incorporation of an additional mutation into the sopB locus of the attenuated sseC, phoP and aroA mutants resulted in the stimulation of improved humoral and cellular immune responses following oral vaccination. The obtained results define a new potential immune escape strategy of this important pathogen, and also demonstrate that this mechanism can be subverted to optimize the immune responses elicited using Salmonella as a live vaccine carrier.",

keywords = "Antigen presentation, Bacterial infection, Dendritic cells, Vaccination",

author = "Claudia Link and Thomas Ebensen and Lothar St{\"a}ndner and Marion D{\'e}josez and Elena Reinhard and Faiza Rharbaoui and Guzm{\'a}n, {Carlos A.}",

note = "Funding Information: Part of this work has been supported by a grant from the European Community (QLK2-CT-1999-00310).",

year = "2006",

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doi = "10.1016/j.micinf.2006.04.013",

language = "English",

volume = "8",

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T1 - An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains

AU - Link, Claudia

AU - Ebensen, Thomas

AU - Ständner, Lothar

AU - Déjosez, Marion

AU - Reinhard, Elena

AU - Rharbaoui, Faiza

AU - Guzmán, Carlos A.

N1 - Funding Information: Part of this work has been supported by a grant from the European Community (QLK2-CT-1999-00310).

PY - 2006/7

Y1 - 2006/7

N2 - Salmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivatives. Cells infected with the double sseC sopB, phoP sopB and aroA sopB mutants also exhibited higher expression of MHC, CD80, CD86 and CD54 molecules, and showed a stronger capacity to process and present an I-Ed-restricted epitope from the influenza hemagglutinin (HA) to CD4+ cells from TCR-HA transgenic mice in vitro. The incorporation of an additional mutation into the sopB locus of the attenuated sseC, phoP and aroA mutants resulted in the stimulation of improved humoral and cellular immune responses following oral vaccination. The obtained results define a new potential immune escape strategy of this important pathogen, and also demonstrate that this mechanism can be subverted to optimize the immune responses elicited using Salmonella as a live vaccine carrier.

AB - Salmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivatives. Cells infected with the double sseC sopB, phoP sopB and aroA sopB mutants also exhibited higher expression of MHC, CD80, CD86 and CD54 molecules, and showed a stronger capacity to process and present an I-Ed-restricted epitope from the influenza hemagglutinin (HA) to CD4+ cells from TCR-HA transgenic mice in vitro. The incorporation of an additional mutation into the sopB locus of the attenuated sseC, phoP and aroA mutants resulted in the stimulation of improved humoral and cellular immune responses following oral vaccination. The obtained results define a new potential immune escape strategy of this important pathogen, and also demonstrate that this mechanism can be subverted to optimize the immune responses elicited using Salmonella as a live vaccine carrier.

KW - Antigen presentation

KW - Bacterial infection

KW - Dendritic cells

KW - Vaccination

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ER -

An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains

Abstract

Keywords

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