@article{fc2789fb337c43c1a9c849197a0d41df,
title = "An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters",
abstract = "The solute carrier (SLC) superfamily represents the biggest family of transporters with important roles in health and disease. Despite being attractive and druggable targets, the majority of SLCs remains understudied. One major hurdle in research on SLCs is the lack of tools, such as cell-based assays to investigate their biological role and for drug discovery. Another challenge is the disperse and anecdotal information on assay strategies that are suitable for SLCs. This review provides a comprehensive overview of state-of-the-art cellular assay technologies for SLC research and discusses relevant SLC characteristics enabling the choice of an optimal assay technology. The Innovative Medicines Initiative consortium RESOLUTE intends to accelerate research on SLCs by providing the scientific community with high-quality reagents, assay technologies and data sets, and to ultimately unlock SLCs for drug discovery.",
keywords = "SLC, cell-based assay, chemical screening, drug discovery, solute carrier, transporters",
author = "Vojtech Dvorak and Tabea Wiedmer and Alvaro Ingles-Prieto and Patrick Altermatt and Helena Batoulis and Felix B{\"a}renz and Eckhard Bender and Daniela Digles and Franz D{\"u}rrenberger and Heitman, {Laura H.} and IJzerman, {Adriaan P.} and Kell, {Douglas B.} and Stefanie Kickinger and Daniel K{\"o}rz{\"o} and Philipp Leippe and Thomas Licher and Vania Manolova and Riccardo Rizzetto and Francesca Sassone and Lia Scarabottolo and Avner Schlessinger and Vanessa Schneider and Sijben, {Hubert J.} and Steck, {Anna Lena} and Hanna Sundstr{\"o}m and Sara Tremolada and Maria Wilhelm and {Wright Muelas}, Marina and Diana Zindel and Steppan, {Claire M.} and Giulio Superti-Furga",
note = "Funding Information: Assay technologies presented here are applied and developed further in the RESOLUTE consortium, a public-private partnership funded by the Innovative Medicines Initiative (IMI) of the European Union. RESOLUTE aims at empowering the research community with open-access reagents and data to unlock the SLC family for drug discovery. One of the main goals of RESOLUTE is to systematically assess the suitability of transport assay technologies for individual SLCs and develop them further (Superti-Furga et al., 2020). We are expecting to update this review with experience gained throughout the project. Funding Information: This work is part of the RESOLUTE project that has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under Grant Agreement No. 777372. This Joint Undertaking receives support from the European Union{\textquoteright}s Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Dvorak, Wiedmer, Ingles-Prieto, Altermatt, Batoulis, B{\"a}renz, Bender, Digles, D{\"u}rrenberger, Heitman, IJzerman, Kell, Kickinger, K{\"o}rz{\"o}, Leippe, Licher, Manolova, Rizzetto, Sassone, Scarabottolo, Schlessinger, Schneider, Sijben, Steck, Sundstr{\"o}m, Tremolada, Wilhelm, Wright Muelas, Zindel, Steppan and Superti-Furga.",
year = "2021",
month = aug,
day = "10",
doi = "10.3389/fphar.2021.722889",
language = "English",
volume = "12",
journal = "Frontiers in Pharmacology",
issn = "1663-9812",
publisher = "Frontiers Media S.A.",
}