TY - JOUR
T1 - An integrated genetic and cytogenetic map for the Mediterranean fruit fly, Ceratitis capitata, based on microsatellite and morphological markers
AU - Stratikopoulos, Elias E.
AU - Augustinos, Antonios A.
AU - Petalas, Yannis G.
AU - Vrahatis, Michael N.
AU - Mintzas, Anastasios
AU - Mathiopoulos, Konstantinos D.
AU - Zacharopoulou, Antigone
N1 - Funding Information:
Acknowledgments We thank I. Papaggeli and K.Oikonomou for support in genotyping and in situ hybridization and G. Franz and J. Cladera for providing medfly strains. E. E. S. was supported by a Fellowship from the National Fellowship Foundation (Greece) and K. D. M. by a grant from European Committee, contract No ERB-FMBICT 983067. This work was supported by grants from the Hellenic General Secretariat of Research and Technology (97EL-17) and University of Patras, Greece. The two anonymous reviewers are greatly appreciated for their useful suggestions.
PY - 2008/6
Y1 - 2008/6
N2 - A genetic map based on microsatellite polymorphisms and visible mutations of the Mediterranean fruit fly (medfly), Ceratitis capitata is presented. Genotyping was performed on single flies from several backcross families. The map is composed of 67 microsatellites and 16 visible markers distributed over four linkage groups. Fluorescence in situ hybridization of selected microsatellite markers on salivary gland polytene chromosomes allowed the alignment of these groups to the second, fourth, fifth and sixth chromosome. None of the markers tested showed segregation either with the X or the third chromosome. However, this map constitutes a substantial starting point for a detailed genetic map of C. capitata. The construction of an integrated map covering the whole genome should greatly facilitate genetic studies and future genome sequence projects of the species.
AB - A genetic map based on microsatellite polymorphisms and visible mutations of the Mediterranean fruit fly (medfly), Ceratitis capitata is presented. Genotyping was performed on single flies from several backcross families. The map is composed of 67 microsatellites and 16 visible markers distributed over four linkage groups. Fluorescence in situ hybridization of selected microsatellite markers on salivary gland polytene chromosomes allowed the alignment of these groups to the second, fourth, fifth and sixth chromosome. None of the markers tested showed segregation either with the X or the third chromosome. However, this map constitutes a substantial starting point for a detailed genetic map of C. capitata. The construction of an integrated map covering the whole genome should greatly facilitate genetic studies and future genome sequence projects of the species.
KW - Ceratitis capitata
KW - Cytological map
KW - Genetic map
KW - Medfly
KW - Microsatellites
UR - http://www.scopus.com/inward/record.url?scp=43349092797&partnerID=8YFLogxK
U2 - 10.1007/s10709-007-9195-9
DO - 10.1007/s10709-007-9195-9
M3 - Article
C2 - 17786564
AN - SCOPUS:43349092797
SN - 0016-6707
VL - 133
SP - 147
EP - 157
JO - Genetica
JF - Genetica
IS - 2
ER -