TY - JOUR
T1 - An in vivo-in vitro comparison of the effects of bile acids on the structural organization and functional activity of liver microsomal monooxygenases
AU - Tsyrlov, Ilya B.
AU - Zakharova-Polyakova, Nataly E.
AU - Gromova, Olga A.
AU - Pospelova, Ludmyla N.
AU - Lyakhovich, Vyacheslav V.
PY - 1978/10
Y1 - 1978/10
N2 - The study deals with changes in the binding and metabolism of type I (aminopyrine and 3,4-benzpyrene) and type II (aniline) substrates by liver microsomal monooxygenases, the activity of NADPH-dependent reductases and lipid peroxidation systems in microsomes, and with the kinetics of ANS binding to microsomal membranes during the development of cholestasis produced by bile duct ligation. The changes observed were compared with the effects of di- and trihydroxy bile acids added to the control microsomal suspension in vitro. It was found that, in contrast with cholic acid, deoxycholic acid (both were sodium salts) added at final concentrations of 0.06-0.07% and 0.09-0.12%, simulates changes in the liver microsomal fraction on 4th and 7th day of cholestasis development, respectively. The corresponding conjugated derivatives of the bile acids used have been characterized by much less effect upon microsomal monooxygenases.
AB - The study deals with changes in the binding and metabolism of type I (aminopyrine and 3,4-benzpyrene) and type II (aniline) substrates by liver microsomal monooxygenases, the activity of NADPH-dependent reductases and lipid peroxidation systems in microsomes, and with the kinetics of ANS binding to microsomal membranes during the development of cholestasis produced by bile duct ligation. The changes observed were compared with the effects of di- and trihydroxy bile acids added to the control microsomal suspension in vitro. It was found that, in contrast with cholic acid, deoxycholic acid (both were sodium salts) added at final concentrations of 0.06-0.07% and 0.09-0.12%, simulates changes in the liver microsomal fraction on 4th and 7th day of cholestasis development, respectively. The corresponding conjugated derivatives of the bile acids used have been characterized by much less effect upon microsomal monooxygenases.
UR - http://www.scopus.com/inward/record.url?scp=0018083845&partnerID=8YFLogxK
U2 - 10.1016/0014-4800(78)90033-3
DO - 10.1016/0014-4800(78)90033-3
M3 - Article
C2 - 99329
AN - SCOPUS:0018083845
SN - 0014-4800
VL - 29
SP - 131
EP - 143
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
IS - 2
ER -