An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening

Fatima Amanat; Kris M. White; Lisa Miorin; Shirin Strohmeier; Meagan McMahon; Philip Meade; Wen Chun Liu; Randy A. Albrecht; Viviana Simon; Luis Martinez-Sobrido; Thomas Moran; Adolfo García-Sastre; Florian Krammer

doi:10.1002/cpmc.108

An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening

Fatima Amanat
, Kris M. White
, Lisa Miorin
, Shirin Strohmeier
, Meagan McMahon
, Philip Meade
, Wen Chun Liu
, Randy A. Albrecht
, Viviana Simon
, Luis Martinez-Sobrido
, Thomas Moran
, Adolfo García-Sastre
, Florian Krammer

Research output: Contribution to journal › Article › peer-review

143 Scopus citations

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro.

Original language	English
Article number	e108
Journal	Current Protocols in Microbiology
Volume	58
Issue number	1
DOIs	https://doi.org/10.1002/cpmc.108
State	Published - 1 Sep 2020

Keywords

COVID-19
COVID19
SARS-CoV-2
antivirals
medium-throughput screening
microneutralization
neutralization

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10.1002/cpmc.108

Cite this

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abstract = "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro.",

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AU - Miorin, Lisa

AU - Strohmeier, Shirin

AU - McMahon, Meagan

AU - Meade, Philip

AU - Liu, Wen Chun

AU - Albrecht, Randy A.

AU - Simon, Viviana

AU - Martinez-Sobrido, Luis

AU - Moran, Thomas

AU - García-Sastre, Adolfo

AU - Krammer, Florian

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An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening

Abstract

Keywords

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