An improved method for the preparation of renaturable apohemoglobin chains

Fred K. Friedman; Kenneth Alston; Alan N. Schechter

doi:10.1016/0003-2697(81)90698-9

An improved method for the preparation of renaturable apohemoglobin chains

Fred K. Friedman, Kenneth Alston, Alan N. Schechter

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A rapid, effective procedure for the preparation of renaturable globin chains from human hemoglobin is described. The method utilizes hydrophobic interaction chromatography in an acid medium to separate the α and β chains of apohemoglobin. Rapid procedures for reconstitution of hemoglobin from these chains and hemin are also presented.

Original language	English
Pages (from-to)	103-107
Number of pages	5
Journal	Analytical Biochemistry
Volume	117
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(81)90698-9
State	Published - Oct 1981
Externally published	Yes

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title = "An improved method for the preparation of renaturable apohemoglobin chains",

abstract = "A rapid, effective procedure for the preparation of renaturable globin chains from human hemoglobin is described. The method utilizes hydrophobic interaction chromatography in an acid medium to separate the α and β chains of apohemoglobin. Rapid procedures for reconstitution of hemoglobin from these chains and hemin are also presented.",

author = "Friedman, {Fred K.} and Kenneth Alston and Schechter, {Alan N.}",

year = "1981",

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doi = "10.1016/0003-2697(81)90698-9",

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T1 - An improved method for the preparation of renaturable apohemoglobin chains

AU - Friedman, Fred K.

AU - Alston, Kenneth

AU - Schechter, Alan N.

PY - 1981/10

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N2 - A rapid, effective procedure for the preparation of renaturable globin chains from human hemoglobin is described. The method utilizes hydrophobic interaction chromatography in an acid medium to separate the α and β chains of apohemoglobin. Rapid procedures for reconstitution of hemoglobin from these chains and hemin are also presented.

AB - A rapid, effective procedure for the preparation of renaturable globin chains from human hemoglobin is described. The method utilizes hydrophobic interaction chromatography in an acid medium to separate the α and β chains of apohemoglobin. Rapid procedures for reconstitution of hemoglobin from these chains and hemin are also presented.

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U2 - 10.1016/0003-2697(81)90698-9

DO - 10.1016/0003-2697(81)90698-9

M3 - Article

C2 - 7316185

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SN - 0003-2697

VL - 117

SP - 103

EP - 107

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

An improved method for the preparation of renaturable apohemoglobin chains

Abstract

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