An improved apparatus for perfusion fixation with automatic pressure control

Joan Gil, Ewald R. Weibel

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A method and equipment are described which allows vascular‐perfusion fixation for electron microscopy under controlled pressure conditions. Hydrostatic pressure can be kept constant during fixation by automatic adjustment of the flow rate of a peristaltic pump which is governed by a fully automatic remote‐control device. Colloid osmotic pressure of the perfusates must also be adjusted to assure adequate balance of the pressures in the tissue at physiological levels. 1971 Blackwell Science Ltd

Original languageEnglish
Pages (from-to)241-244
Number of pages4
JournalJournal of Microscopy
Volume94
Issue number3
DOIs
StatePublished - Dec 1971
Externally publishedYes

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