An HPLC-UV method for the simultaneous quantification of curcumin and its metabolites in plasma and lung tissue: Potential for preclinical applications

Jagdish Mahale, Lynne M. Howells, Rajinder Singh, Robert G. Britton, Hong Cai, Karen Brown

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Curcumin, derived from turmeric, has been extensively investigated for its broad spectrum of biological activities. Previously reported HPLC-UV methods have focussed on analysis of the parent compound. Here, a sensitive HPLC-UV method was developed and partially validated, then used for the simultaneous determination of curcumin and its glucuronide and sulfate metabolites in plasma and lung tissue from mice. The assay was applied to an in vivo pharmacokinetic study comparing formulated curcumin (Meriva™) with standard curcumin. Plasma levels of glucuronide and sulfate metabolites were 5- and 2-fold higher after Meriva™ administration compared with standard curcumin. In lung tissue, free curcumin was 4-fold higher following Meriva™ administration vs standard curcumin. This assay represents a rapid, cheap method for simultaneous detection of curcumin and its major metabolites that has applicability in pre-clinical settings.

Original languageEnglish
Article numbere4280
JournalBiomedical Chromatography
Volume32
Issue number9
DOIs
StatePublished - Sep 2018
Externally publishedYes

Keywords

  • HPLC-UV
  • curcumin
  • curcumin glucuronide
  • curcumin sulfate
  • lung cancer

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