TY - JOUR
T1 - An enzyme-linked immunosorbent assay (Elisa) for the detection of monoclonal antibodies to cell surface antigens on viable cells
AU - Posner, Marshall R.
AU - Antoniou, Demetri
AU - Griffin, James
AU - Schlossman, Stuart F.
AU - Lazarus, Herbert
N1 - Funding Information:
We would like to thank Dr. Lee M. Nadler for the use of monoclonal antibodies I-1 and fi-2 microglobulin, Dr. John Pesando and Kevin Tomaselli for the use of G/M 12sI, Herbert Levine for making the G/M FITC and Dr. D. Golde for the use of the KG-1 cell line. Dr. Griffin is supported in part by the Johanna C. Wood Foundation and the Medical Foundation.
Funding Information:
Supported in part by NIH Grants CA 09172, CA 19126, CA 19589. Address reprint requests to: Dr. Marshall R. Posner, Sidney Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, U.S.A.
PY - 1982/1/15
Y1 - 1982/1/15
N2 - A rapid and inexpensive ELISA method is described which is suitable for the large scale screening of monoclonal antibodies to cell surface antigens. The use of acrylic plates and viable cells eliminates background and false positive reactions, and avoids modification of surface antigens caused by fixation. This facilitates easy and rapid detection of positives by visual inspection of the plates. The specificity and sensitivity of the method is comparable to indirect immunofluorescence or radioimmunoassay. The advantages of this ELISA system when compared to these methods and to previously described ELISA systems utilizing fixed cells are discussed.
AB - A rapid and inexpensive ELISA method is described which is suitable for the large scale screening of monoclonal antibodies to cell surface antigens. The use of acrylic plates and viable cells eliminates background and false positive reactions, and avoids modification of surface antigens caused by fixation. This facilitates easy and rapid detection of positives by visual inspection of the plates. The specificity and sensitivity of the method is comparable to indirect immunofluorescence or radioimmunoassay. The advantages of this ELISA system when compared to these methods and to previously described ELISA systems utilizing fixed cells are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0020062298&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(82)90206-X
DO - 10.1016/0022-1759(82)90206-X
M3 - Article
C2 - 7035567
AN - SCOPUS:0020062298
SN - 0022-1759
VL - 48
SP - 23
EP - 31
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -