@article{4004dd447ed044d099256bc0fe725e0a,
title = "An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells",
abstract = "Growing evidence implicates the importance of glia, particularly astrocytes, in neurological and psychiatric diseases. Here, we describe a rapid and robust method for the differentiation of highly pure populations of replicative astrocytes from human induced pluripotent stem cells (hiPSCs), via a neural progenitor cell (NPC) intermediate. We evaluated this protocol across 42 NPC lines (derived from 30 individuals). Transcriptomic analysis demonstrated that hiPSC-astrocytes from four individuals are highly similar to primary human fetal astrocytes and characteristic of a non-reactive state. hiPSC-astrocytes respond to inflammatory stimulants, display phagocytic capacity, and enhance microglial phagocytosis. hiPSC-astrocytes also possess spontaneous calcium transient activity. Our protocol is a reproducible, straightforward (single medium), and rapid (<30 days) method to generate populations of hiPSC-astrocytes that can be used for neuron-astrocyte and microglia-astrocyte co-cultures for the study of neuropsychiatric disorders.",
keywords = "astrocyte, human induced pluripotent stem cell, iPSC",
author = "Julia TCW and Minghui Wang and Pimenova, {Anna A.} and Bowles, {Kathryn R.} and Hartley, {Brigham J.} and Emre Lacin and Machlovi, {Saima I.} and Rawan Abdelaal and Karch, {Celeste M.} and Hemali Phatnani and Slesinger, {Paul A.} and Bin Zhang and Goate, {Alison M.} and Brennand, {Kristen J.}",
note = "Funding Information: BV2 cell line was kindly provided by Marc Diamond (UT Southwestern Medical Center). We are grateful for the continuous support of the Flow Cytometry Core at the Icahn School of Medicine at Mount Sinai Hospital. This work was supported in part by the JPB Foundation (A.M.G.), the Rainwater Foundation (A.M.G.), NIMH R01MH101454 (K.J.B.), NIA U01P50AG005138-30-1 (Alzheimer's Disease Research Center: Pilot 30-1) (K.J.B.), NIA/NIH U01AG046170 (K.J.B., M.W., and B.Z.), a component of the AMP-AD Target Discovery and Preclinical Validation Project, NIMH R01MH11499 (P.A.S.), NIAAA R01AA018734 (P.A.S.), NIDA R01DA037170 (P.A.S.), K01AG046374 (C.M.K.), and the New York Stem Cell Foundation (K.J.B.) and Project ALS (H.P.). A.M.G. serves on the Scientific Advisory Board for Denali Therapeutics. Publisher Copyright: {\textcopyright} 2017 The Authors",
year = "2017",
month = aug,
day = "8",
doi = "10.1016/j.stemcr.2017.06.018",
language = "English",
volume = "9",
pages = "600--614",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "2",
}