TY - JOUR
T1 - An EAR-motif-containing ERF transcription factor affects herbivore-induced signaling, defense and resistance in rice
AU - Lu, Jing
AU - Ju, Hongping
AU - Zhou, Guoxin
AU - Zhu, Chuanshu
AU - Erb, Matthias
AU - Wang, Xiaopeng
AU - Wang, Peng
AU - Lou, Yonggen
PY - 2011/11
Y1 - 2011/11
N2 - Ethylene responsive factors (ERFs) are a large family of plant-specific transcription factors that are involved in the regulation of plant development and stress responses. However, little to nothing is known about their role in herbivore-induced defense. We discovered a nucleus-localized ERF gene in rice (Oryza sativa), OsERF3, that was rapidly up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis. Antisense and over-expression of OsERF3 revealed that it positively affects transcript levels of two mitogen-activated protein kinases (MAPKs) and two WRKY genes as well as concentrations of jasmonate (JA), salicylate (SA) and the activity of trypsin protease inhibitors (TrypPIs). OsERF3 was also found to mediate the resistance of rice to SSB. On the other hand, OsERF3 was slightly suppressed by the rice brown planthopper (BPH) Nilaparvata lugens (Stål) and increased susceptibility to this piercing sucking insect, possibly by suppressing H 2O 2 biosynthesis. We propose that OsERF3 affects early components of herbivore-induced defense responses by suppressing MAPK repressors and modulating JA, SA, ethylene and H 2O 2 pathways as well as plant resistance. Our results also illustrate that OsERF3 acts as a central switch that gears the plant's metabolism towards an appropriate response to chewing or piercing/sucking insects.
AB - Ethylene responsive factors (ERFs) are a large family of plant-specific transcription factors that are involved in the regulation of plant development and stress responses. However, little to nothing is known about their role in herbivore-induced defense. We discovered a nucleus-localized ERF gene in rice (Oryza sativa), OsERF3, that was rapidly up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis. Antisense and over-expression of OsERF3 revealed that it positively affects transcript levels of two mitogen-activated protein kinases (MAPKs) and two WRKY genes as well as concentrations of jasmonate (JA), salicylate (SA) and the activity of trypsin protease inhibitors (TrypPIs). OsERF3 was also found to mediate the resistance of rice to SSB. On the other hand, OsERF3 was slightly suppressed by the rice brown planthopper (BPH) Nilaparvata lugens (Stål) and increased susceptibility to this piercing sucking insect, possibly by suppressing H 2O 2 biosynthesis. We propose that OsERF3 affects early components of herbivore-induced defense responses by suppressing MAPK repressors and modulating JA, SA, ethylene and H 2O 2 pathways as well as plant resistance. Our results also illustrate that OsERF3 acts as a central switch that gears the plant's metabolism towards an appropriate response to chewing or piercing/sucking insects.
KW - WRKY
KW - defense-related signaling
KW - ethylene responsive factor
KW - herbivore resistance
KW - mitogen-activated protein kinase
KW - rice
UR - http://www.scopus.com/inward/record.url?scp=80855130714&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2011.04709.x
DO - 10.1111/j.1365-313X.2011.04709.x
M3 - Article
C2 - 21831212
AN - SCOPUS:80855130714
SN - 0960-7412
VL - 68
SP - 583
EP - 596
JO - Plant Journal
JF - Plant Journal
IS - 4
ER -