An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation

Bo Huo, Xin L. Lu, Kevin D. Costa, Qiaobing Xu, X. Edward Guo

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

To investigate the roles of intercellular gap junctions and extracellular ATP diffusion in bone cell calcium signaling propagation in bone tissue, in vitro bone cell networks were constructed by using microcontact printing and self-assembled monolayer technologies. In the network, neighboring cells were interconnected through functional gap junctions. A single cell at the center of the network was mechanically stimulated by using an AFM nanoindenter. Intracellular calcium ([Ca2+]i) responses of the bone cell network were recorded and analyzed. In the untreated groups, calcium propagation from the stimulated cell to neighboring cells was observed in 40% of the tests. No significant difference was observed in this percentage when the intercellular gap junctions were blocked. This number, however, decreased to 10% in the extracellular ATP-pathway-blocked group. When both the gap junction and ATP pathways were blocked, intercellular calcium waves were abolished. When the intracellular calcium store in ER was depleted, the indented cell can generate calcium transients, but no [Ca2+]i signal can be propagated to the neighboring cells. No [Ca2+]i response was detected in the cell network when the extracellular calcium source was removed. These findings identified the biochemical pathways involved in the calcium signaling propagation in bone cell networks.

Original languageEnglish
Pages (from-to)234-241
Number of pages8
JournalCell Calcium
Volume47
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

Keywords

  • AFM indentation
  • ATP
  • Gap junction
  • Intracellular calcium store
  • Osteoblast

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