TY - JOUR
T1 - An acetyltransferase-independent function of Eso1 regulates centromere cohesion
AU - Lin, Su Jiun
AU - Tapia-Alveal, Claudia
AU - Jabado, Omar J.
AU - Germain, Doris
AU - O'Connell, Matthew J.
N1 - Funding Information:
We thank Jean-Paul Javerzat for providing strains and the anti-K106 acetyl-Psm3 antibody and gratefully acknowledge Emily Outwin for the construction of eso1-1. This work was supported by National Institute of General Medical Sciences/National Institutes of Health Grant R01-GM088162 and National Cancer Institute/National Institutes of Health Grant T32-CA078207.
Publisher Copyright:
© 2016 Lin et al.
PY - 2016/12/15
Y1 - 2016/12/15
N2 - Eukaryotes contain three essential Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin, and Smc5/6. Cohesin forms a ring-shaped structure that embraces sister chromatids to promote their cohesion. The cohesiveness of cohesin is promoted by acetylation of N-terminal lysines of the Smc3 subunit by the acetyltransferases Eco1 in Saccharomyces cerevisiae and the homologue, Eso1, in Schizosaccharomyces pombe. In both yeasts, these acetyltransferases are essential for cell viability. However, whereas nonacetylatable Smc3 mutants are lethal in S. cerevisiae, they are not in S. pombe. We show that the lethality of a temperature-sensitive allele of eso1 (eso1-H17) is due to activation of the spindle assembly checkpoint (SAC) and is associated with premature centromere separation. The lack of cohesion at the centromeres does not correlate with Psm3 acetylation or cohesin levels at the centromeres, but is associated ith significantly reduced recruitment of the cohesin regulator Pds5. The SAC activation in this context is dependent on Smc5/6 function, which is required to remove cohesin from chromosome arms but not centromeres. The mitotic defects caused by Smc5/6 and Eso1 dysfunction are cosuppressed in double mutants. This identifies a novel function (or functions) for Eso1 and Smc5/6 at centromeres and extends the functional relationships between these SMC complexes.
AB - Eukaryotes contain three essential Structural Maintenance of Chromosomes (SMC) complexes: cohesin, condensin, and Smc5/6. Cohesin forms a ring-shaped structure that embraces sister chromatids to promote their cohesion. The cohesiveness of cohesin is promoted by acetylation of N-terminal lysines of the Smc3 subunit by the acetyltransferases Eco1 in Saccharomyces cerevisiae and the homologue, Eso1, in Schizosaccharomyces pombe. In both yeasts, these acetyltransferases are essential for cell viability. However, whereas nonacetylatable Smc3 mutants are lethal in S. cerevisiae, they are not in S. pombe. We show that the lethality of a temperature-sensitive allele of eso1 (eso1-H17) is due to activation of the spindle assembly checkpoint (SAC) and is associated with premature centromere separation. The lack of cohesion at the centromeres does not correlate with Psm3 acetylation or cohesin levels at the centromeres, but is associated ith significantly reduced recruitment of the cohesin regulator Pds5. The SAC activation in this context is dependent on Smc5/6 function, which is required to remove cohesin from chromosome arms but not centromeres. The mitotic defects caused by Smc5/6 and Eso1 dysfunction are cosuppressed in double mutants. This identifies a novel function (or functions) for Eso1 and Smc5/6 at centromeres and extends the functional relationships between these SMC complexes.
UR - http://www.scopus.com/inward/record.url?scp=85006341654&partnerID=8YFLogxK
U2 - 10.1091/mbc.E16-08-0596
DO - 10.1091/mbc.E16-08-0596
M3 - Article
C2 - 27798241
AN - SCOPUS:85006341654
SN - 1059-1524
VL - 27
SP - 4002
EP - 4010
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 25
ER -