Amplification of T cells from human cord blood in serum-deprived culture stimulated with stem cell factor, interleukin-7 and interleukin-2

M. Sanchez, E. Alfani, A. R. Migliaccio, T. Bonfini, G. Migliaccio

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We report the effects exerted by cytokine combinations, including stem cell factor (SCF), interleukin-7, interleukin-4 and interleukin-2, on the amplification of T cells from cord blood (CB) mononuclear cells cultured for 10-11 days under serum-deprived conditions. Of all the combinations investigated, SCF + interleukin-7 sustained the best fold increase (FI) of total nucleated cells (FI = 6.4±1.17), amplifying preferentially CD4+ over CD8+ T-cell subsets (FI = 4.72±0.79 vs 2.73±1.2, respectively, P < 0.05). The addition of interleukin-2 to this combination did not significantly increase the total number of cells generated (FI = 7.4±2.27), but allowed preferential amplification of CD8+ over CD4+ T cells (FI = 6.04±0.14 vs 1.67±0.6, respectively, P < 0.05). Single-strand conformation polymorphism analysis of the T-cell receptor Vβ-chain rearrangements expressed by the expanded T cells indicated that the complexity of the T-cell repertoire had increased after 10 days of culture in the presence of SCF and IL-7. Interestingly, a modest expansion (FI = 8.67±1.5 of myeloid progenitor cells was also observed in these cultures. These results indicate that it is possible to expand specific T-cell subsets for adoptive immunotherapy without losing myeloid progenitor cells necessary for neutrophil recovery after CB transplantation, by modulating the cytokines added to the cultures.

Original languageEnglish
Pages (from-to)713-723
Number of pages11
JournalBone Marrow Transplantation
Volume31
Issue number8
DOIs
StatePublished - Apr 2003
Externally publishedYes

Keywords

  • Cord blood
  • Ex vivo amplification
  • Interleukin-2
  • Interleukin-7
  • Serum-free cultures
  • Stem cell factor
  • T lymphocytes

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