TY - JOUR
T1 - Amplification, expression, and packaging of a foreign gene by influenza virus
AU - Luytjes, Willem
AU - Krystal, Mark
AU - Enami, Masayoshi
AU - Parvin, Jeffrey D.
AU - Palese, Peter
N1 - Funding Information:
We are most thankful to Kazue Enami for her skillful assistance during the course of the experiments. This work was supported by Merit Award Al-18998 (P. P) and by Public Health Service grants Al-2663 (M. K.) and Al-24460 and Al-11823 (P P) from the National Institutes of Health. M. K. was also supported by funds from the New York Lung Association and an Irma T. Hirschl Scholar Award. J. D. l? was a trainee on Medical Scientist Research Grant GM07280 from the NIH.
PY - 1989/12/22
Y1 - 1989/12/22
N2 - A system is described that allows use of recombinant DNA technology to modify the genome of influenza virus, a negative-strand RNA virus, and to engineer vectors for the expression of foreign genes. Recombinant RNA is expressed from plasmid DNA in which the coding sequence of the influenza A virus NS gene is replaced with that of the chloramphenicol acetyltransferase gene. When transfected with purified influenza A virus polymerase proteins-in the presence of helper virus-the recombinant RNA is amplified, expressed, and packaged into virus particles, which can be passaged several times. The data indicate that the 22 5′ terminal and the 26 3′ terminal bases of the influenza A virus RNA are sufficient to provide the signals for RNA transcription, RNA replication, and packaging of RNA into influenza virus particles.
AB - A system is described that allows use of recombinant DNA technology to modify the genome of influenza virus, a negative-strand RNA virus, and to engineer vectors for the expression of foreign genes. Recombinant RNA is expressed from plasmid DNA in which the coding sequence of the influenza A virus NS gene is replaced with that of the chloramphenicol acetyltransferase gene. When transfected with purified influenza A virus polymerase proteins-in the presence of helper virus-the recombinant RNA is amplified, expressed, and packaged into virus particles, which can be passaged several times. The data indicate that the 22 5′ terminal and the 26 3′ terminal bases of the influenza A virus RNA are sufficient to provide the signals for RNA transcription, RNA replication, and packaging of RNA into influenza virus particles.
UR - http://www.scopus.com/inward/record.url?scp=0024846089&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(89)90766-6
DO - 10.1016/0092-8674(89)90766-6
M3 - Article
C2 - 2598262
AN - SCOPUS:0024846089
SN - 0092-8674
VL - 59
SP - 1107
EP - 1113
JO - Cell
JF - Cell
IS - 6
ER -