Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients

E. Premkumar Reddy; Magnhild Sandberg-Wollheim; Richard V. Mettus; Phillip E. Ray; Elaine DeFreitas; Hilary Koprowski

doi:10.1126/science.2536193

Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients

E. Premkumar Reddy, Magnhild Sandberg-Wollheim, Richard V. Mettus, Phillip E. Ray, Elaine DeFreitas, Hilary Koprowski

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.

Original language	English
Pages (from-to)	529-533
Number of pages	5
Journal	Science
Volume	243
Issue number	4890
DOIs	https://doi.org/10.1126/science.2536193
State	Published - 1989
Externally published	Yes

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title = "Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients",

abstract = "Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.",

author = "Reddy, {E. Premkumar} and Magnhild Sandberg-Wollheim and Mettus, {Richard V.} and Ray, {Phillip E.} and Elaine DeFreitas and Hilary Koprowski",

year = "1989",

doi = "10.1126/science.2536193",

language = "English",

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journal = "Science",

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T1 - Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients

AU - Reddy, E. Premkumar

AU - Sandberg-Wollheim, Magnhild

AU - Mettus, Richard V.

AU - Ray, Phillip E.

AU - DeFreitas, Elaine

AU - Koprowski, Hilary

PY - 1989

Y1 - 1989

N2 - Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.

AB - Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.

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VL - 243

SP - 529

EP - 533

JO - Science

JF - Science

IS - 4890

ER -

Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients

Abstract

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