Amperometric phenol biosensor based on polyaniline

Peng Wang, Min Liu, Jinqing Kan

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


The catechol biosensor is constructed by cross-linking between polyphenol oxidase (PPO) and polyaniline (PANI) using glutaraldehyde as a cross-linking agent. The PANI, which is electrochemically synthesized in a solution containing ionic liquid, 1-ethyl-3-methylimidazolium ethyl sulfate, possesses good electroactivity and high conductivity above pH 6. In the presence of catechol as a substrate, the biosensor exhibits a linear range from 0.2 to 80 μmol dm-3. The maximum response current (Imax) and the Michaelis-Menten constant (km) are 9.44 μA and 117 μmol dm-3, respectively. The effects of pH and operating potential are also explored to optimize measurement conditions. The activation energy (Ea) of the PPO catalytic reaction is 30.23 kJ mol-1 in the B-R buffer. Electrochemical impedance spectroscopy (EIS), UV-vis and SEM are used to characterize the PANI-PPO biosensor. The biosensor exhibits good long-term stability.

Original languageEnglish
Pages (from-to)577-584
Number of pages8
JournalSensors and Actuators B: Chemical
Issue number2
StatePublished - 16 Jul 2009
Externally publishedYes


  • Biosensor
  • Catechol
  • Cross-linking process
  • Ionic liquid
  • Polyaniline
  • Polyphenol oxidase


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