Altered methotrexate sensitivity in human leukemic K562 amino acid transport mutant cells

Kevin J. Scanlon; Rona Riegelhaupt; Michèle Paliai; Holly Kisthard; Sharon Kassan; Samuel Waxman

Altered methotrexate sensitivity in human leukemic K562 amino acid transport mutant cells

Kevin J. Scanlon
, Rona Riegelhaupt
, Michèle Paliai
, Holly Kisthard
, Sharon Kassan
, Samuel Waxman

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Evidence is presented for a human mutant cell line (K562-M) which possesses altered amino acid membrane transport properties with different methotrexate (MTX) sensitivity from its respective parent cell line (K562-P). This was not due to any significant change in the amount of the dihydrofolate reductase. The initial uptake of MTX into both cell lines (K562P and K562M) was also similar. This mutant cell line demonstrates the following characteristics: 1. A two fold resistance in MTX cytotoxicity. 2. An apparent difference in either the Kt and/or Vmax for specific amino acid substrates and 3. Increased inhibition of amino acid transport by MTX.

Original language	English
Title of host publication	St. Andrews, Scotland, September 21-24, 1982
Publisher	de Gruyter
Pages	933-939
Number of pages	7
ISBN (Electronic)	9783111619453
ISBN (Print)	9783110085600
State	Published - 6 Feb 2015

Cite this

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title = "Altered methotrexate sensitivity in human leukemic K562 amino acid transport mutant cells",

abstract = "Evidence is presented for a human mutant cell line (K562-M) which possesses altered amino acid membrane transport properties with different methotrexate (MTX) sensitivity from its respective parent cell line (K562-P). This was not due to any significant change in the amount of the dihydrofolate reductase. The initial uptake of MTX into both cell lines (K562P and K562M) was also similar. This mutant cell line demonstrates the following characteristics: 1. A two fold resistance in MTX cytotoxicity. 2. An apparent difference in either the Kt and/or Vmax for specific amino acid substrates and 3. Increased inhibition of amino acid transport by MTX.",

author = "Scanlon, \{Kevin J.\} and Rona Riegelhaupt and Mich{\`e}le Paliai and Holly Kisthard and Sharon Kassan and Samuel Waxman",

note = "Publisher Copyright: {\textcopyright} 1983 Walter de Gruyter \& Co., Berlin • New York.",

year = "2015",

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TY - CHAP

T1 - Altered methotrexate sensitivity in human leukemic K562 amino acid transport mutant cells

AU - Scanlon, Kevin J.

AU - Riegelhaupt, Rona

AU - Paliai, Michèle

AU - Kisthard, Holly

AU - Kassan, Sharon

AU - Waxman, Samuel

PY - 2015/2/6

Y1 - 2015/2/6

N2 - Evidence is presented for a human mutant cell line (K562-M) which possesses altered amino acid membrane transport properties with different methotrexate (MTX) sensitivity from its respective parent cell line (K562-P). This was not due to any significant change in the amount of the dihydrofolate reductase. The initial uptake of MTX into both cell lines (K562P and K562M) was also similar. This mutant cell line demonstrates the following characteristics: 1. A two fold resistance in MTX cytotoxicity. 2. An apparent difference in either the Kt and/or Vmax for specific amino acid substrates and 3. Increased inhibition of amino acid transport by MTX.

AB - Evidence is presented for a human mutant cell line (K562-M) which possesses altered amino acid membrane transport properties with different methotrexate (MTX) sensitivity from its respective parent cell line (K562-P). This was not due to any significant change in the amount of the dihydrofolate reductase. The initial uptake of MTX into both cell lines (K562P and K562M) was also similar. This mutant cell line demonstrates the following characteristics: 1. A two fold resistance in MTX cytotoxicity. 2. An apparent difference in either the Kt and/or Vmax for specific amino acid substrates and 3. Increased inhibition of amino acid transport by MTX.

UR - https://www.scopus.com/pages/publications/85140278072

M3 - Chapter

AN - SCOPUS:85140278072

SN - 9783110085600

SP - 933

EP - 939

BT - St. Andrews, Scotland, September 21-24, 1982

PB - de Gruyter

ER -

Altered methotrexate sensitivity in human leukemic K562 amino acid transport mutant cells

Abstract

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